在环境矩阵中的聚乙烯纳米颗粒的定量分离,具有使用毛细体电泳术检测极限的图形图
Michael A Caprise1, Ana C Quevedo2, Kathryn R Riley1
1Department of Chemistry & Biochemistry, Swarthmore College, 500 College Ave, Swarthmore, PA 19081, USA. kriley1@swarthmore.edu.
概括
我们开发了一种毛细电泳方法,通过尺寸或表面特性来分离聚乙烯颗粒 (PSP). 这种技术有效地在15分钟内解决了PSP在各种解决方案中.
科学领域:
- 分析化学 分析化学
- 材料科学 材料科学 材料科学
背景情况:
- 聚乙烯颗粒 (PSP) 在各种应用中广泛使用.
- 基于物理和化学性质的PSP的有效分离对于它们的表征和应用至关重要.
研究的目的:
- 开发一种毛细电泳 (CE) 方法,用于分离聚乙烯颗粒.
- 为了证明该方法在解决不同尺寸和表面功能的PSP方面的有效性.
主要方法:
- 毛细管电泳用于粒子分离.
- 分离是在含有粘土或苏瓦尼河酸的缓冲溶液中进行的,度为100毫克L-1.
主要成果:
- 开发的CE方法实现了PSP的基线或接近基线的解决.
- 对于尺寸和表面功能不同的PSP,证明了有效的分离.
- 在所有测试条件下,分辨率在15分钟内实现.
结论:
- 毛细管电泳提供了一种快速有效的方法来分离聚乙烯颗粒.
- 该CE方法是稳固的,适用于存在的环境矩阵,如粘土和酸.
更多相关视频
相关概念视频
Capillary Electrophoresis: Applications
401
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
401
Electrophoresis: Overview
2.0K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
2.0K
Capillary Electrophoresis: Instrumentation
237
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
237
Size-Exclusion Chromatography
606
In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
Silica particles offer advantages such as rigidity,...
Silica particles offer advantages such as rigidity,...
606


