阿斯特拉加卢斯多糖化物通过激活STAT5/IGF-1通路来改善HepG2细胞中的胰岛素耐药性
Xinxin Yue1, Wei Hao1, Min Wang1
1Department of Clinical College, HE University, Shenyang, Liaoning, China.
Immunity, inflammation and disease
|November 29, 2023
概括
阿斯特拉加卢斯多糖 (APC) 通过激活STAT5/IGF-1通路,改善肝细胞中的胰岛素抵抗 (IR). 这种传统中医成分增强葡萄糖吸收和细胞活力,为2型糖尿病治疗提供了潜力.
科学领域:
- 生物化学 生物化学
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
背景情况:
- 胰岛素抵抗 (IR) 是2型糖尿病的一个关键因素,影响肝脏的葡萄糖吸收.
- 众所周知,从传统中医药中提取的阿斯特拉加卢斯多糖 (APC) 抑制红外线,但其机制尚不清楚.
研究的目的:
- 调查阿斯特拉加卢斯多糖 (APC) 改善 HepG2 肝细胞胰岛素抵抗 (IR) 的潜在机制.
- 探索STAT5/IGF-1信号通路在APC对IR的影响中的作用.
主要方法:
- 在诱导胰岛素抵抗 (IR) 后,HepG2细胞被用APC治疗.
- 评估了细胞活力,葡萄糖吸收和关键蛋白质 (IGF-1,IGF-1R,p-STAT5/STAT5,p-AKT/AKT) 的表达.
- 使用AG490研究了STAT5的作用,AG490是一种STAT5信号抑制剂.
主要成果:
- 治疗APC增加了HepG2细胞活力,并恢复了IR抑制的葡萄糖吸收.
- APC使IGF-1R,IGF-1的表达和AKT和STAT5通路的激活正常化.
- 通过AG490减少了APC的有益作用,证实了STAT5.5的参与.
结论:
- 阿斯特拉加卢斯多糖 (APC) 有效地改善 HepG2 细胞中的胰岛素抵抗 (IR).
- 该机制涉及到STAT5信号通路的激活,导致增强IGF-1信号.
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