不同代的Mycoplasma synoviae对胚胎的致病性和免疫作用
1School of Animal Science and Technology, Ningxia University, Yinchuan, Ningxia, China.
British poultry science
|November 29, 2023
概括
在胚胎中,Mycoplasma synoviae (MS) 的致病性随着通道增加而降低. 热冲击蛋白 (HSP) 和炎症基因表达发生了变化,这表明疫苗开发可能会减弱.
科学领域:
- 兽医免疫学 兽医免疫学
- 鸟类病理学 鸟类病理学
- 分子生物学分子生物学
背景情况:
- 神经质突菌 (MS) 是家禽突炎的关键原因之一.
- 了解MS的致病性和免疫反应对于疾病控制至关重要.
研究的目的:
- 通过连续通道,研究Mycoplasma synoviae (MS) 在特定无病原体 (SPF) 胚胎中的致病性和免疫反应.
- 分析热冲击蛋白 (HSP) 和炎症因素的死亡率和基因表达.
主要方法:
- 在7天大的SPF胚胎中连续传递MS (F1到F200).
- 死亡率的记录和分析.
- 逆转录量聚合酶连锁反应 (RT-qPCR) 针对气管组织中的HSP (HSP27,HSP40,HSP60,HSP70,HSP90) 和炎症因子 (IL-1β,caspase-1,IL-18).
主要成果:
- 随着MS的传递增加,死亡率大幅下降,从F1的80%降至F200的10%.
- 随着通道的增加,HSP27,IL-1β,HSP40,caspase-1,HSP70和HSP90的表达显著下调 (P <0.05).
- 随着通道的增加,HSP60表达显著上升 (P <0.05),而IL-18没有显著的趋势.
结论:
- 在胚胎中,MS病原性和通道数之间存在明显的关系.
- 降低的致病性与HSP和炎症基因的改变表达有关.
- 这些发现为选择多发性硬化菌株提供了潜在的弱化疫苗开发的基础.
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