昆虫杀虫剂菲普罗尼尔在Chironomus riparius中调节分子反应的环境相关度
Thandy Junio da Silva Pinto1,2, José-Luis Martínez-Guitarte3, Mariana Amaral Dias2
1PPG-SEA and NEEA/CRHEA/SHS, São Carlos Engineering School, University of São Paulo, São Carlos, Brazil.
Environmental toxicology and chemistry
|November 29, 2023
概括
即使杀虫剂fipronil的环境度很低,也会显著改变水生昆虫幼虫的基因表达和酶活性. 这种农药影响了Chironomus riparius的多个细胞通路,突出显示了它对淡水生态系统的潜在危害.
科学领域:
- 环境毒理学环境毒理学
- 生态毒理学 生态毒理学
- 分子生物学分子生物学
背景情况:
- 像fipronil这样的杀虫剂污染水生环境,对水生动物构成风险.
- 菲普罗尼尔是一种广泛的杀虫剂,主要向昆虫的神经系统,但可以通过其他机制影响非向生物.
研究的目的:
- 为了评估fipronil对基因表达和酶活性在Chironomus riparius幼虫的亚生物的影响.
- 为了研究环境相关的fipronil度对关键水生无脊椎动物模型的影响.
主要方法:
- 短期毒性测试暴露Chironomus riparius幼虫在24小时和96小时内暴露在fipronil (0.0010.1μg L-1).
- 用实时聚合酶链反应分析42个基因的信使RNA水平.
- 测量催化酶 (CAT),谷氨S转移酶 (GST) 和乙胆酶 (AChE) 酶活动.
主要成果:
- 96小时后,fipronil暴露改变了参与内分泌,解毒,压力和免疫反应的基因.
- 菲普罗尼尔在0.01微克L-1时增加了CAT和GST活性,并在96小时后在更高度下增加了ACHE活性.
- 没有观察到对幼虫死亡率的显著影响,但发生了实质性的分子调制.
结论:
- 环境相关的fipronil度可以调节Chironomus riparius中的分子反应,即使在短期暴露后也是如此.
- 亚生物体的变化表明fipronil对淡水生物的有害影响,强调生物标志物对早期损害检测的有用性.
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