粘弹性分类集成变形性细胞计用于高通量分类和高精度机械型化瘤细胞
Yao Chen1, Lin Jiang1, Xiaozhe Zhang1
1School of Mechanical Engineering, Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China.
Analytical chemistry
|November 29, 2023
概括
一种新的粘弹性排序集成变形性细胞计 (VSDC) 能够实现循环瘤细胞 (CTC) 的高通量,无标签的隔离和机械表型. 这项技术准确地将瘤细胞与背景血细胞区分开来,以改善癌症监测.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 循环瘤细胞 (CTC) 是癌症监测和预后的关键生物标志物.
- 在液体活检中,区分CTC与丰富的,外观相似的血细胞是一个重大挑战.
- 基于标签的方法可以改变细胞特性,并且通常具有低通量.
研究的目的:
- 开发一种高通量,无标签的方法,从全血中分离和机械表型瘤细胞.
- 为了克服背景血细胞干扰在CTC分析中的局限性.
- 为了能够精确地机械地表征单个瘤细胞.
主要方法:
- 将用于移除大型背景细胞的粘弹性分类芯片和用于机械性质分析的检测芯片集成到单一设备 (VSDC) 中.
- 基于粘弹性特性进行无标签分类.
- 多个细胞机械参数的高精度测量.
- 机器学习算法应用于用于细胞识别的机械数据.
主要成果:
- VSDC实现了超过95%的分类效率和超过81%的瘤细胞纯度.
- 机械参数的机器学习分析使瘤细胞与白细胞的准确分化成为可能.
- 整体瘤细胞识别准确率超过97%,MCF-7细胞的识别准确率达到99.4%.
结论:
- VSDC提供了一个强大的工具,用于高通量,无标签的单细胞分析.
- 这项技术为改善癌症诊断和研究中CTC隔离和表征提供了有前途的方法.
- VSDC在各种生物医学领域具有潜在的应用,需要精确的细胞分析.
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