接近红外线的光线不会导致人类皮肤纤维细胞的DNA受损
Jessica Mineroff1,2, Jennifer Y Wang1,2, Raichel Philip1
1Department of Dermatology, SUNY Downstate Health Sciences University, Brooklyn, New York, USA.
Journal of biophotonics
|November 29, 2023
概括
近红外 (NIR) 光生物调制 (PBM) 疗法利用特定波长的光来促进愈合. 这项研究发现,NIR光,通常用于皮肤病学,不会导致人类皮肤细胞的DNA损伤,确保其治疗应用的安全性.
科学领域:
- 皮肤病学 皮肤病学
- 照相医疗 (photomedicine) 是一种医学.
- 分子生物学分子生物学
背景情况:
- 光生物调制 (PBM) 使用红色和近红外 (NIR) 光进行皮肤病治疗.
- 光辐射对DNA的损伤有助于皮肤衰老和癌症.
- 之前的研究证实红光 (RL) PBM不会损害人类皮肤纤维细胞 (HDF) DNA.
研究的目的:
- 研究近红外 (NIR) 光生物调节在人类皮肤纤维细胞 (HDF) 中诱导DNA损伤的潜力.
- 通过评估其基因毒性作用,确保NIR光疗在皮肤病应用中的安全性.
主要方法:
- 人体皮肤纤维细胞 (HDF) 用商业可用的LED-NIR面板在不同能量密度 (66,132,264 J/cm2) 处进行辐射.
- 通过量化循环butan 胺二聚物 (CPD) 和胺-6,4-胺光产物 (6-4PPs) 来评估DNA损伤.
- 在辐射后3小时和24小时立即分析HDF,并与温度匹配的阳性和阴性对照进行比较.
主要成果:
- 在任何测试能量密度的NIR照射后,在HDF中没有观察到使用CPD和6-4PP测量的显著DNA损伤.
- 辐射后的所有时间点 (即时,3小时,24小时) 的结果一致.
- 与对照组相比,没有发现DNA损伤标志物的统计学上显著差异.
结论:
- 商用LED近红外 (NIR) 光在测试参数上不会在体外诱导人类皮肤纤维细胞中的DNA损伤.
- 在皮肤病学应用中,NIR光生物调制疗法被证明是安全的,关于皮肤病学应用的基因毒性.
- 进一步的研究可以自信地探索NIR PBM在皮肤病学中的治疗益处.
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