通过影响辅酶介导调节,BRIP1和BRIP2维持根髓系
Xin Song1, Yaoguang Yu1, Jiameng Zhu1
1State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Planta
|November 29, 2023
概括
在BRAHMA相互作用蛋白1和2 (BRIP1/2) 中的突变通过影响辅酶运输和下调PLETHORA (PLT) 基因来破坏根部发育. 这影响了根髓系统的维护和干细胞利基组织.
科学领域:
- 植物生物学 植物生物学
- 发展生物学 发展生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 交换缺陷/非发酵糖 (SWI/SNF) 染色质重塑复合物对于植物发育至关重要.
- 与BRAHMA (BRM) 相关的SWI/SNF复合体 (BAS) 子单元BRAHMA相互作用蛋白1 (BRIP1) 和BRIP2参与植物发育,但它们在根部发育中的作用尚不清楚.
研究的目的:
- 调查BRIP1和BRIP2在根髓系统发育中的功能.
- 阐明根部发育的BRIP1/2调节背后的分子机制.
主要方法:
- 对brip1 brip2双突变和plt1 plt2四重突变的分析.
- 染色体免疫沉 (ChIP) 来评估与PIN基因结合的BRIP1/2.
- 德甲 (DEX) 诱导的PLT2转基因线来评估救援效应.
主要成果:
- brip1 brip2突变体表现出缩短的根髓系统和无组织的干细胞.
- BRIP1/2突变会降低PIN基因的表达,损害auxin运输和渐变.
- BRIP1/2直接与PIN基因结合,并降低PLT基因表达的调节.
结论:
- 通过通过PIN基因调节auxin运输,BRIP1/2对根介质系统发育至关重要.
- 部分通过PLT1/2通道发挥BRIP1/2的功能,以维持根干细胞种群.
- 这些发现揭示了SWI/SNF复合体在植物根部发育中的新机制.
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