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相关实验视频

Updated: Jul 9, 2025

Ex Vivo Perfusion of the Rodent Placenta
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精密切割切片培养方法用于老鼠胎盘

Fusun Gundogan1,2, Jeffrey Gilligan3, Suzanne de la Monte4,5,6

  • 1Regional Perinatal Pathology, Kaiser Permanente Oakland Medical Center, Oakland, CA, USA. fusun.x.gundogan@kp.org.

Methods in molecular biology (Clifton, N.J.)
|November 29, 2023
PubMed
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有机型切片培养保留了复杂的组织特征,比传统的细胞模型提供了优势. 本研究详细介绍了一种用于研究应用的制造大鼠胎盘有机型切片培养的方法.

科学领域:

  • * 生殖生物学 生殖生物学
  • * 发育毒理学 * 发育毒理学
  • * 组织工程是组织工程.

背景情况:

  • * 传统的细胞培养模型 (单层) 通常无法复制复杂的体内组织环境.
  • *有机型切片培养保存了三维 (3D) 组织结构,多系细胞种群和复杂的功能属性.
  • *这些培养物作为研究组织对各种药物的反应和预测体内结果的有价值模型.

研究的目的:

  • * 提出一种标准化的方法来制备来自老鼠胎盘的器官切片培养.
  • *强调这些作物的实用性,作为生殖和毒理学研究中的研究模型.

主要方法:

  • * 切割和切割大鼠胎盘组织的详细程序.
  • * 建立维持胎盘器官类切片活力和结构完整性的条件.
  • * 切片的准备,用于随后的暴露和分析.

主要成果:

  • *成功地从老鼠胎盘中生成了可行的器官类型切片培养物.
  • * 在准备好的切片中证明了胎盘关键结构和细胞特征的保留.
  • * 建立了利用这些培养在实验研究的基础.

结论:

关键词:
方法论 方法论 方法论机体类型的器官类型.胎盘 胎盘 胎盘 胎盘切片文化是一种切片文化.热细胞 热细胞

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Last Updated: Jul 9, 2025

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  • * 鼠胎盘的有机切片培养是一种可行的和有利的模型系统.
  • * 这种方法有助于研究胎盘生物学和对异生菌的反应.
  • *与传统的细胞培养技术相比,这些培养提供了更好的预测模型.