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Updated: Jul 9, 2025

A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
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基于阿克里迪尼的化学发光受体-连接体结合试验对蛋白质/激素进行测试

André Guilherme Daubermann1, Keini Dressano1,2, Paulo Henrique de Oliveira Ceciliato1,2

  • 1Laboratório de Bioquímica de Proteínas, Departamento de Ciências Biológicas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo (ESALQ/USP), Piracicaba, Brazil.

Methods in molecular biology (Clifton, N.J.)
|November 29, 2023
PubMed
概括

化学发光的阿克里迪尼 Ester 提供一种非放射性,高效的方法来标记蛋白质和. 这种技术可以在联体结合试验中进行特定检测,为分子研究中的传统放射性标记提供了可靠的替代方案.

关键词:
埃科林 (Aequorin) 是一种象牙.具有约束力的测定.在Ca2+调动试验中进行测试.化学发光的光泽在HPLC净化过程中.类标签的标签 类标签酸净化净化方法受体-连接体相互作用.

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