相关实验视频

Updated: Jul 9, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

PRIME EDITING - 一种编辑基因的新方法

Dalia Blicharska¹, Izabela Szućko-Kociuba², Ewa Filip³

¹Instytut Biologii, Uniwersytet Szczeciński, Szczecin. dalia.blicharska.9@gmail.com.

Postepy biochemii

|November 29, 2023

概括

总编辑是一个新的基因编辑工具,可以精确地修改DNA,而不需要双链断裂. 这种先进的CRISPR/Cas9衍生品在植物,动物和人类细胞中提供了精确的基因组编辑.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
生物技术是生物技术.

背景情况:

克里斯普尔/卡斯9技术彻底改变了基因组编辑.
克里斯普尔/卡斯9的局限性包括双链断裂和非目标效应.
首席编辑 (PE) 作为对CRISPR/Cas9.9的进步而出现.

研究的目的:

描述主要编辑系统的开发和改进.
详细介绍Prime Editing的结构和功能.
审查目前在各种生物体中使用Prime Editing的研究.

主要方法:

使用Cas9-尼克酶与逆转录酶融合.
使用一个主要编辑指导RNA (pegRNA),其中包含反转录的模板.
不会诱导双链DNA断裂,与传统的CRISPR/Cas9.9不同.

主要成果:

主编辑允许精确的插入,删除和基础替换.
在植物,动物和人类细胞基因组中表现出有效性.
成功修改了高精度的目标DNA序列.

结论:

总编辑代表了基因组编辑技术的重大进步.
与以前的方法相比,提供了更高的精度和多功能性.
具有治疗应用和遗传研究的巨大潜力.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

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