转录因子MdBPC2通过调节auxin生物合成,改变果生长并促进化
Haiyan Zhao1, Shuyuan Wan1, Yanni Huang1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling, 712100 Shaanxi, China.
The Plant cell
|November 29, 2023
概括
一种新的果转录因子MdBPC2通过调节auxin生物合成来控制植物结构. 这一发现提供了在多年木质植物中基因改进矮根茎的潜力.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 农业科学 农业科学
背景情况:
- 辅酶对植物生长和发育至关重要,但其在植物结构中的调节机制尚未完全理解.
- 矮人根茎在多年木质植物中至关重要,用于管理大小和提高产量.
研究的目的:
- 鉴定和描述涉及果 (Malus × domestica) 辅酶调节和植物结构的转录因子.
- 阐明这种转录因子影响auxin生物合成和植物形态的分子机制,特别是在矮矮的根茎中.
主要方法:
- 在果中识别和表达BBR/BPC家族转录因子 (MdBPC2) 的分析.
- 在果中进行过度表达和基因沉默实验,以评估表型效应和auxin水平.
- 生物化学测试以确定MdBPC2与辅酶合成基因促进体 (MdYUC2a,MdYUC6b) 的相互作用.
- 研究MdBPC2与Polycomb组蛋白LHP1的相互作用及其在表观遗传修饰 (H3K27me3) 中的作用.
主要成果:
- 在矮小的根茎和负调节的辅酶生物合成中,MdBPC2的表达高.
- 过度表达MdBPC2导致植物高度降低,叶子形态变化,根部发育受阻,这些表型与素水平降低有关.
- MdBPC2直接与MdYUC2a和MdYUC6b促进体中的GAGA丰富元素结合,抑制它们的转录.
- MdBPC2与MdLHP1相互作用,通过H3K27me3抑制基因表达,这一过程通过沉默MdLHP1.1而逆转.
结论:
- MdBPC2作为辅酶生物合成的关键调节剂,有助于果根茎中矮化表型.
- MdBPC2-LHP1复合体调解了辅酶合成基因的表观遗传沉默,提供了一种控制植物结构的新机制.
- 这一发现表明MdBPC2是果根茎基因改进的潜在目标,以控制植物的大小和结构.
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