居民微生物塑造了阴道上皮质甘氨酸的景观
Kavita Agarwal1,2,3,4, Biswa Choudhury4, Lloyd S Robinson1,2
1Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110, USA.
Science translational medicine
|November 29, 2023
概括
细菌性阴道炎 (BV) 改变阴道上皮质糖,减少保护性糖并暴露潜在的银河糖. 来自像Gardnerella这样的BV相关细菌的酶可能会驱动这些变化,影响表皮细胞的反应.
科学领域:
- 微生物学 微生物学
- 葡萄糖生物学 葡萄糖生物学
- 皮质生物学 皮质生物学
背景情况:
- 阴道上皮肤表面覆盖着一种保护性葡萄糖,一种碳水化合物 (glycans) 的层.
- 细菌性阴道炎 (BV) 与负面的健康结果有关,涉及阴道细菌与上皮直接接触.
- 阴道上皮的特定甘氨酸组成及其在BV中的变化尚未得到充分了解.
研究的目的:
- 为了描述正常的阴道上皮质糖景观.
- 调查寄居微生物对阴道葡萄糖的影响,特别是在BV的背景下.
- 探索细菌甘酸酸酶在改变阴道甘的形状中的作用.
主要方法:
- 电子显微镜可视化葡萄糖的结构.
- 生物化学和质谱测量以分析甘氨酸成分.
- 用重组*Gardnerella* sialidases治疗原发性阴道上皮细胞和培养的VK2细胞.
主要成果:
- 与对照组相比,BV样本显示了明显减少的葡萄糖.
- 在BV上皮细胞中缺少化N-和O-甘氨酸,暴露了银河糖残留物.
- *Gardnerella* 化剂治疗诱导了类似于BV的糖变化和与细胞死亡,分化和炎症相关的基因表达变化.
结论:
- 在B.V.中,阴道上皮质甘氨酸景观发生了显著的改变.
- 细菌 sialidases 可能有助于观察到的 BV. glycan 变化.
- 改变的上皮细胞甘氨酸和细菌酶可能会影响上皮细胞循环和宿主微生物群相互作用.
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