来自儿科人口的鼻和便样本中的SARS-CoV-2分泌动力学
Haifa Khemiri1,2, Mariem Gdoura1,2, Samar Ben Halima1,2
1Laboratory of Clinical Virology, WHO Regional Reference Laboratory for Poliomyelitis and Measles for the EMR, Institut Pasteur de Tunis, University of Tunis El Manar, Tunis, Tunisia.
Frontiers in medicine
|November 29, 2023
概括
严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) RNA在有症状和无症状儿童的便中分泌长达14天. 然而,感染性SARS-CoV-2并没有从便样本中分离出来,这表明儿童的便-口腔传播风险有限.
科学领域:
- 病毒学 病毒学
- 儿童传染病 儿童传染病
- 公共卫生 公共卫生
背景情况:
- 严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 可以导致儿童的胃肠道症状.
- 口腔-便传播是SARS-CoV-2感染的可疑途径.
- 了解儿童的病毒泄漏对于感染控制至关重要.
研究的目的:
- 确定儿童鼻和便样本中SARS-CoV-2RNA泄露的持续时间和发生率.
- 在感染儿童的便样本中调查SARS-CoV-2的生存能力.
主要方法:
- 实时RT-PCR被用于检测来自71名儿童的鼻和便样本中的SARS-CoV-2RNA.
- 在阳性样本上进行了全基因组测序.
- 从便样本使用Vero细胞培养物进行病毒分离试验.
主要成果:
- 在所有71名儿童的鼻样本和15名儿童的便样本中检测到SARS-CoV-2RNA (21.1%).
- 便分泌发生在有症状和无症状的儿童中,持续长达14天.
- 已经确定了四种SARS-CoV-2变种 (阿尔法,B.1.160,三角形,奥米克朗).
结论:
- 儿童可以在呼吸道和便样本中长时间分泌SARS-CoV-2RNA.
- 尽管在便中检测到RNA,但无法隔离传染性病毒,这表明便-口腔传播的风险很低.
- 需要进一步的研究,以充分阐明便在儿童群体SARS-CoV-2传播动态中的作用.
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