光生物调节可以增强有听力神经病变的耳中的干细胞活力,但不能恢复听力
So-Young Chang1, Eunjeong Kim2, Nathaniel T Carpena3
1Beckman Laser Institute Korea, Dankook University, Cheonan 31116, Republic of Korea.
Stem cells international
|November 29, 2023
概括
用小鼠胚胎干细胞 (mESC) 和光生物调制 (PBM) 进行的联合治疗没有在二次神经病变模型中恢复听力. 然而,PBM显著增加了无恶性耳组织内的干细胞增殖和分布.
科学领域:
- 再生医学是一种再生医学.
- 神经科学是一个神经科学.
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
背景情况:
- 感觉神经听力损失带来了重大的治疗挑战.
- 耳植入物提供康复,但依赖于听觉神经细胞的完整性.
- 二次性神经病理模型对于研究听力损失治疗至关重要.
研究的目的:
- 研究小鼠胚胎干细胞 (mESC) 和光生物调制 (PBM) 对二次神经病变动物模型中的听觉功能和神经细胞的联合作用.
- 为了评估mESC和PBM组合疗法的疗效,kanamycin诱导的耳损伤模型.
主要方法:
- 建立了一种使用卡纳米辛 (KM) 治疗的二次神经病变动物模型.
- 同培养的GFP标记的mESC与KM处理的耳扩展剂一起,以确认移植.
- 对动物模型进行GFP-mESC和近红外PBM的联合治疗.
- 通过听觉脑干响应 (ABR) 和电脑听力学 (eABR) 评估听力恢复.
- 通过光分析评估形态变化和干细胞分布.
主要成果:
- 卡纳米辛治疗将听力值提高了70-80dB.
- 联合mESC和PBM疗法没有恢复听觉功能.
- 干细胞移植导致耳干细胞数量的指数增长,由于PBM,没有观察到恶性瘤.
- 在耳组织中,PBM治疗促进了干细胞的广泛分布和活跃增殖,与细胞迅速丢失的对照组不同.
结论:
- 在这个模型中,mESC和PBM治疗的结合并没有改善听力或增强听觉神经反应.
- 光生物调节显著提高了在尾细胞中移植的干细胞的存活和增殖.
- 进一步的研究可能会探索优化PBM参数或细胞类型,以获得听力损失的潜在治疗益处.
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