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大麻烟在小鼠中抑制了对流感A的抗病毒免疫反应
Nadia Milad1,2,3,4, Matthew F Fantauzzi1,2,3,4, Joshua J C McGrath5
1Department of Medicine, McMaster University, Hamilton, ON, Canada.
ERJ open research
|November 29, 2023
概括
暴露于大麻烟雾增加了小鼠肺部的甲型流感病毒 (IAV),减少了免疫细胞和炎症信号. 这表明吸食大麻可能会损害对病毒感染的免疫反应.
科学领域:
- 免疫学 免疫学 免疫学
- 呼吸系统医学 呼吸系统医学
- 药理学 药理学是指药理学的学科.
背景情况:
- 大麻使用量正在增加,但其对流感A病毒 (IAV) 等病毒感染的影响尚不清楚.
- 与香烟烟雾相比,大麻素的抗炎性质表明大麻烟可能独特地影响抗病毒反应.
研究的目的:
- 在小鼠模型中调查日常大麻烟雾暴露对宿主免疫机制在流感A病毒感染期间的影响.
- 确定大麻烟雾如何影响病毒负担,免疫细胞种群,炎症媒介和抗体反应.
主要方法:
- 雄性和雌性BALB/c小鼠暴露在大麻烟雾中,并感染了IAV.
- 评估肺病毒载量,炎症调解剂 (ELISA),肺免疫细胞 (流细胞计) 和基因表达 (RNA测序).
- 通过ELISA测量了血IAV特异性抗体 (IgM,IgG1).
主要成果:
- 暴露于大麻烟雾会增加肺部病毒负担,并减少白细胞,巨细胞,单细胞和树突细胞.
- 炎症介导体 (IFN-γ,CXCL5) 被削弱;女性表现出下调的免疫反应途径.
- 在暴露于大麻的雌性小鼠中,IAV特异性IgM和IgG1抗体水平降低.
结论:
- 暴露于大麻烟雾会破坏宿主防御,增加病毒载量,并在IAV感染期间减轻炎症.
- 结果表明,大麻吸烟在病毒感染期间对肺部平衡有害.
- 需要进一步研究大麻吸烟对人类免疫能力的影响.
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