相关实验视频

Updated: Jul 9, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

通过合成染色体及其组合重构来建立染色体设计-构建-测试-学习

Jee Loon Foo^1,2,3,4, Shohei Kitano^1,2,3,4, Adelia Vicanatalita Susanto^1,2,3,4

¹NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), National University of Singapore, Singapore 117456, Singapore.

|November 29, 2023

概括

研究人员设计了一种合成酵母染色体 (synXV),以加速生物研究. 该系统能够快速进行基因操纵和翻译分析,进步合成生物学和菌株工程.

科学领域:

合成生物学合成生物学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

染色体级设计-构建-测试-学习周期 (chrDBTLs) 提供了系统的染色体重新配置.
合成生物学方法对于理解和设计细胞功能至关重要.

研究的目的:

在Saccharomyces cerevisiae中建立合成染色体XV (synXV),用于高级chrDBTL.
开发用于高效合成染色体操纵和分析的新方法.
用 synXV 来研究基因表达和翻译动态.

主要方法:

重编码合成染色体XV (synXV) 的设计和制造.
对chrDBTL.的CRISPR-Cas9介导的线性重组与内置复制 (CRIMiRE) 的发展.
对synXV和野生类型酵母的核糖体概况,用于位置对位置的翻译比较.

主要成果:

成功建立了synXV,具有战略性插入的特征和重新编码的基因.
为了基因型-表型映射,CRIMiRE 能够快速生成定制酵母菌株.
对比的核糖体占用率分析揭示了代码子变化对体内翻译的影响.

结论:

SynXV 作为一个通用的平台,用于推进 chrDBTL.
开发的方法加速了合成染色体的重新设计和生物发现.
这项工作提供了对翻译动态的洞察,并促进了应变工程.

关键词:

这就是CRISPR-Cas9的特征.这种植物是Saccharomyces cerevisiae.染色体重新配置的染色体重新配置设计-建造-测试-学习线粒体复合的复合方式合成生物学合成生物学合成染色体的人造染色体合成基因组学合成基因组学人造酵母合成酵母

更多相关视频

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

Published on: May 25, 2015

相关概念视频

Synthetic Biology

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...

Chromosome Structure

Chromosome Structure

A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Karyotyping

Karyotyping

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

Crossing Over

Crossing Over

Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...