双-A 干扰荷尔蒙水平和丸线粒体活动,降低男性生育能力
Do-Yeal Ryu1, Won-Ki Pang1, Elikanah Olusayo Adegoke1
1Department of Animal Science & Technology and BET Research Institute, Chung-Ang University, Anseong, Republic of Korea.
Human reproduction open
|November 29, 2023
概括
双甲 (BPA) 暴露会通过改变丸线粒体功能和激素水平来破坏男性生育能力. 这项对小鼠的研究揭示了BPA.
科学领域:
- 内分泌学和生殖毒理学
- 线粒体生物学 线粒体生物学
- 环境健康 环境健康
背景情况:
- 全球越来越关注双A (BPA),需要了解它对男性生殖健康的作用机制.
- 众所周知,BPA通过与雌激素受体 (ERs) 相互作用引起荷尔蒙失衡,但确切的机制仍在研究中.
研究的目的:
- 为了研究双-A (BPA) 对小鼠男性生育能力的影响.
- 阐明BPA暴露激活的特定机制,重点关注激素干扰和线粒体功能.
主要方法:
- 在一项比较研究中,雄性小鼠在六周内接触到BPA的最低观察到的不良影响水平 (LOAEL) (50 mg/kgBW/天).
- 通过ELISA评估荷尔蒙水平 (FSH,LH,),评估丸组织学 (H&E染色),并使用西欧涂抹分析线粒体活性和亡相关蛋白质.
- 研究了暴露于BPA的精子对体外受精 (IVF) 结果的影响,包括裂变和胚胎细胞形成.
主要成果:
- 暴露于BPA激活了蛋白激酶A和基因激活蛋白激酶信号通路,通过与ER和G蛋白结合受体结合.
- 观察到异常激素水平,线粒体呼吸和ATP合成受损,以及丸线粒体中亡的增加.
- 在LOAEL剂量下,减少了胚胎裂变和芽细胞形成率,以及异常的精卵管形态和丸的组织病变.
结论:
- 暴露于BPA会通过激素干扰和对丸线粒体动力学和活动的不良影响显著损害男性生育能力.
- 该研究强调了BPA对丸的特定毒性,即使在剂量不会导致体重显著变化的情况下也是如此.
- 建议使用与人类相关的BPA暴露水平进行进一步的体外研究和调查,以充分了解毒理影响.
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