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结性脊柱炎患者和健康对照患者血液微生物群的分子特征

Dargham Bayan Mohsen Hammad¹, Omar Abdulazeez Alhamad², Alaa Mahdy Obiad Khzal¹

¹Department of Pathological Analyses, Faculty of Science, Kufa University, Najaf, Iraq.

Medical journal of the Islamic Republic of Iran

|November 29, 2023

概括

微生物DNA存在于结性脊髓炎患者和健康个体的血液中. 血液中的特定细菌种类在结性脊髓炎中发生变化,这表明它们可能是疾病的生物标志物.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
遗传学遗传学是一个

背景情况:

结节性脊柱炎 (AS) 越来越多地与微生物组的改变有关,特别是在肠道.
血液循环中的微生物DNA可能作为远程微生物组变化的生物标志物.
以前的研究集中在肠道微生物组,但血液微生物组是新兴的研究领域.

研究的目的:

为了比较AS患者血液中的微生物DNA的存在和身份,与健康对照对比.
为了研究结性脊柱炎的潜在血液生物标志物.
探索特定的血液微生物种群与疾病活动之间的关系.

主要方法:

案例研究设计,比较10名AS患者和10名健康对照.
从血液样本中对细菌16SrRNA基因进行PCR放大和测序.
使用Luminex测定和统计分析的细胞因子TNF-alpha,IL-17A和IL-23的量化与未配对的T-测试和曼-惠特尼测试.

主要成果:

在80%的AS患者和健康对照中检测到微生物16SrRNA.
血液中占主导地位的细菌类包括蛋白质细菌,菌细菌,动菌细菌和细菌类.
亚氏杆菌与显著增加的细菌菌类,Prevotella和Micrococcus有关,并减少了Corynebacterium 1,Gemella和Alloprevotella.
在血液中,Prevotella属与AS患者的TNF-α水平有很强的正相关性.

结论:

在健康个体中存在着独特的血液微生物组.
血液中的特定微生物种类在结性脊髓炎中受到调节.
血液衍生的微生物特征需要进一步研究,作为AS的潜在疾病生物标志物.

关键词:

16S Rrna 基因的使用结性脊柱炎是一种疾病.生物标志物生物标志物血液中的微生物组失生症是一种失生症.

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