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土壤 Alveolata在未受干扰的草原和小麦的多样性在不同的耕作条件下发展

N B Naumova¹, P A Barsukov¹, O A Baturina¹

¹Institute of Soil Science and Agrochemistry of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

Vavilovskii zhurnal genetiki i selektsii

|November 29, 2023

概括

用ITS2的metabarcoding评估了土壤微核细胞的多样性,特别是膜细胞. 与耕种土壤相比,未被干扰的草原土壤显示出更高的状动物多样性和独特的社区,这表明土地使用对多样性的影响比耕作更大.

科学领域:

土壤微生物学土壤微生物学
单核细胞的多样性
分子生态学分子生态学

背景情况:

微核细胞对土壤健康至关重要,但与细菌和真菌相比,它们的研究不足.
气泡体是重要的土壤微生物,充当消费者和猎物.
高通量测序为研究微生物群落提供了新的途径.

研究的目的:

在不同土地使用和耕作实践下,调查西伯利亚土壤中状层的多样性.
为了比较未受干扰的草原和小麦作物田 (传统耕种和无耕种) 之间的土壤状群体.
确定土地利用和耕作对土壤状动物的多样性和丰富性的影响.

主要方法:

从未受到干扰的草原 (美国),传统耕作 (CT) 和无耕作 (NT) 领域的土壤样本中提取了DNA.
内部转录间隔器2 (ITS2) 标记物使用ITS3_KYO2 / ITS4原始器进行了放大.
使用Illumina MiSeq对片进行了测序,并确定了操作分类学单位 (OTU).

主要成果:

总共有198种Alveolata OTU被确定,其中158种属于Ciliophora族.
与CT和NT土壤相比,在未受干扰的草原 (美国) 中,酸盐多样性 (OTU丰富度) 和社区组成 (β-生物多样性) 显著更高,更明显.
特定的类动物 (Litostomatea,Phyllopharyngea) 在美国更为丰富,而Nassophorea和Spirotrichea与美国土壤中的沙子和粘土含量等土壤特性相关.

结论:

土地利用在很大程度上塑造了土壤状动物群落,未被干扰的草原土壤支持了更高的多样性和独特的组合.
与土地利用相比,耕地实践 (CT和NT) 对状动物多样性的影响较小.
这些发现强调了植物材料输入量和质量对影响状动物群落的重要性,并表明需要使用多种omics方法进行进一步的研究.

关键词:

切尔诺泽姆 - 切尔诺泽姆一个ITS区域的ITS区域.状动物状动物状动物传统的耕作方式.没有耕地,没有耕地.

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