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新型的转录组网络与果果的生长适应阻光的温室膜有关

Xin He¹, Celymar A Solis², Sachin G Chavan¹

¹National Vegetable Protected Cropping Centre, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, Australia.

Frontiers in plant science

|November 29, 2023

概括

阻光膜 (LBF) 改变光谱,影响豆果的发育和基因表达. LBFs促进黄蛋白,但减少其他胡卜素,在后期阶段观察到显著的转录基因变化,揭示了低光适应的关键基因.

科学领域:

植物分子生物学和遗传学
被保护的作物和农业技术.
生物化学和植物新陈代谢

背景情况:

阻光膜 (LBF) 在受保护的作物中改变光传输 (UVA,PAR,蓝色,红色光谱),影响植物生长和产量.
了解LBF效应背后的分子机制对于优化豆等作物的保护种植至关重要.

研究的目的:

为了研究色 (Capsicum annuum L.) 果对LBF治疗的转录和代谢反应.
确定关键的基因和代谢途径,参与适应LBF引起的改变光条件.

主要方法:

在LBF治疗和对照下,在授粉 (DAP) 后的35天和65天,对白果的转录组分析 (RNA-Seq).
有针对性的代谢物分析,以评估胡卜素样本的变化.
生物信息分析包括差异基因表达 (DEG) 和聚类以确定关键途径和基因.

主要成果:

在35 DAP时,LBF治疗显著改变了胡卜素样式,增加了黄蛋白,同时减少了山丁和新山丁.
在65 DAP (2,654 DEG) 观察到更多的差异表达基因 (DEG),而不是35 DAP (1,192 DEG).
65 DAP 的关键 DEG 与光信号 (例如,UVR8,PIF4,COP1),植物激素信号 (ABA),粉/糖代谢,胡卜素合成和烯胺生物合成有关.

结论:

在分子层面上,LBF显著影响果果的发育,特别是在晚期的成熟阶段.
参与光感知,激素信号和二次代谢物生物合成的基因对于适应LBF至关重要.
在受保护的作物系统中,已识别的候选基因有可能改善瓜的质量和低光适应性.

关键词:

红 (Capsicum annuum) L. 年 (Capsicum annuum) L. 年 (Capsicum annuum) 是一种年.果子开发果子开发温室覆盖材料可以覆盖玻璃房.光感受器的光感受器低亮度的低亮度是什么意思代谢途径的代谢途径转录组 (transcriptome) 是一个转录组.

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