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微粒子介导的CRISPRDNA传递用于树中的基因组编辑

Lennart Hoengenaert^1,2, Jan Van Doorsselaere³, Ruben Vanholme^1,2

¹Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.

Frontiers in plant science

|November 29, 2023

概括

用DNA涂层的微粒子轰炸使得在树中实现了无转基因的CRISPR/Cas9基因编辑. 这种方法还促进了针对性捐赠者DNA的整合,促进了基因编辑的发展.

科学领域:

植物生物技术植物生物技术
基因组工程是基因组工程.
林业林业林业林业林业

背景情况:

克里斯普尔/Cas9是精确植物基因组工程的首选方法,特别是在树中.
农细菌介导的转化对于树的CRISPR/Cas9传递是常见的,但导致稳定的T-DNA集成.
开发无转基因基因编辑树木是植物生物技术的一个重要目标.

研究的目的:

为了评估DNA涂层的微粒轰炸,用于在树中输送CRISPR/Cas9试剂.
评估产生无转基因,基因编辑树的潜力.
通过这种方法,研究在特定的目标部位整合捐赠者DNA的可行性.

主要方法:

探索DNA涂层微粒轰炸,将CRISPR/Cas9组件输送到Populus tremula x P. alba*中.
优化转化方法,以促进传递基因的短暂表达.
基因编辑植物的再生和对转基因存在和捐赠者DNA集成的分析.

主要成果:

经过基因编辑的再生植物没有*Cas9*和抗生素耐药性转基因.
在Cas9诱导的双链断裂中观察到的供体DNA片段的频繁整合.
通过微粒子轰炸开发无转基因,基因编辑树的潜力已被证明.

结论:

用DNA涂层的微粒子轰炸是无转基因CRISPR/Cas9基因编辑的可行替代方案.
这种方法通过在特定的基因组位点实现捐赠者DNA集成来促进向基因插入.
开辟了开发基因编辑树木的新途径,没有不必要的转基因集成.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?基因编辑基因编辑粒子轰炸粒子轰炸粒子轰炸这是一棵树,树.有针对性的插入.组织培养的组织培养.没有转基因的自由基因.

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