邻近的Intins会干扰彼此的定位能力
Israela Turgeman-Grott1, Danielle Arsenault2, Dekel Yahav1
1The Shmunis School of Biomedicine and Cancer Research, Faculty of Life Sciences, Tel Aviv University, P.O. Box 39040, 6997801 Tel Aviv, Israel.
PNAS nexus
|November 29, 2023
概括
整因是移动的遗传元素. 在Haloarchaea中,微染色体维护 (MCM) 蛋白质.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 微生物学 微生物学
背景情况:
- 整因是生命中发现的移动遗传元素,能够入侵宿主基因.
- 微染色体维护 (MCM) 蛋白质对于DNA复制至关重要,在Haloarchaea中呈现多个整蛋白插入点.
- 在MCM蛋白中,特定的整蛋白插入位 (MCM-a和MCM-d) 经常是目标.
研究的目的:
- 为了研究*Haloferax mediterranei*的MCM蛋白中邻近的intins之间的intin入侵动态和相互作用.
- 分析整个Haloferacales顺序的MCM同类物体中intin插入点的频率和保存情况.
- 为了了解控制在haloarchaeal MCM蛋白中的intin homing和splicing的机制.
主要方法:
- 在*Haloferax mediterranei*中进行体内研究,以观察虫的入侵和拼接.
- 来自129个 Haloferacales 种的 MCM 同类物种的计算分析.
- 在不同的入侵场景下评估Intin识别,分离和定位效率.
主要成果:
- 在MCM-a和MCM-d站点的Intin被识别并割裂他们的目标.
- 单一的intin定位是高效的,但共同定位到没有intin的站点是不那么有效的.
- 当一个 intein 已经在邻近的网站上存在时,入侵更成功.
- 在MCM-d部位的特定氨基酸替代 (氨酸替代氨酸) 阻止了拼接,但不能识别.
结论:
- 在 haloarchaeal MCM 蛋白质中,因特因的入侵和回归表现出复杂的动态,受到邻近因特因的存在的影响.
- 整因追溯的效率取决于上下文,有利于已经部分入侵过的等位基因的入侵.
- 特定的extein残留物可以调节intein拼接而不取消intein识别,突出复杂的宿主-intein相互作用.
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