处理的二氧化促进了蛋白质甘氨酸层的形成,改变了骨髓干细胞的化和免疫耐受能力
Tomomi Mizutani1, Shuhei Tsuchiya1, Masaki Honda2
1Department of Oral Maxillofacial Surgery, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi Mizuho-cho Mizuho-ku, Nagoya, Aichi, 467-8602, Japan.
Biochemistry and biophysics reports
|November 29, 2023
概括
对二氧化 (TiO2) 表面的性处理增强了蛋白质甘油 (PGs) 层的形成,改善了人类骨髓中介质干细胞 (hBMSCs) 的骨质生成和免疫耐受性. 这种修改促进了TiO2植入物上更好的化和干细胞行为.
科学领域:
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 蛋白质甘油 (PGs) 在骨再生和细胞矩阵相互作用中起着至关重要的作用.
- 二氧化 (TiO2) 在牙科和骨科植入物中广泛使用,但优化其骨质整合和免疫调节特性仍然是一个挑战.
- 性处理是一种已知的方法,可以增强TiO2表面的骨质生成.
研究的目的:
- 研究性处理对TiO2表面对蛋白质甘油 (PG) 层形成的影响.
- 分析性处理的TiO2对人类骨髓介质干细胞 (hBMSCs) 的骨质生成能力和免疫耐受性的影响.
- 为了确定PG层的变化是否介导性处理的观察到的效果.
主要方法:
- 处理 (Ti-Al) 和未处理 (Ti-MS) 表面的表面拓和湿度的表征.
- 评估hBMSC的行为,包括细胞附着,增殖,化,性酸酶活性和PGS层的形成.
- 在两个表面培养的hBMSC中分析骨质生和免疫耐受性基因表达.
- 从hBMSCs对巨细胞 (hMps) 和Jurkat细胞免疫耐受性基因表达的条件介质 (CM) 的评估.
主要成果:
- 与Ti-MS相比,Ti-Al上的hBMSCs表现出增强的细胞附着,增殖,PG层形成和骨质生成能力.
- 在培养在Ti-Al.Al上的hBMSC中观察到增加的类胺2,3-二氧化酶 (IDO) 基因表达.
- 从hBMSCs对Ti-Al的条件介质改变了Jurkat细胞中的基因表达,表明免疫调节效应,但没有显著影响巨细胞极化.
结论:
- 对TiO2表面的性处理显著改变了PGS层的形成.
- 在处理的TiO2上修改的PGs层增强了hBMSCs的骨质生成和免疫耐受性.
- 这些发现表明,性处理是改善基于TiO2的植入物的生物相容性和疗效的有希望的策略.
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