肌肉结构的生物机械刺激通过调节肌分泌来影响骨结构的表型
Harshini Suresh Kumar1, Edwina N Barnett1, John L Fowlkes2,3
1Department of Biomedical Engineering University of Kentucky Lexington KY USA.
JBMR plus
|November 29, 2023
概括
高强度间歇训练 (HIIT) 在体外刺激肌肉细胞以改善模拟糖尿病中的葡萄糖吸收和骨健康. 这项研究为代谢性骨病提供了新的治疗点.
科学领域:
- 生物医学工程 生物医学工程
- 代谢性骨病研究研究
- 糖尿病的肌肉骨并发症
背景情况:
- 糖尿病会导致糖尿病肌肉病和骨疾病,肌肉与骨的相互作用还不太清楚.
- 运动训练显示出预防和保护这些肌肉骨并发症的前景.
- 肌肉分泌物显著影响骨生物学,这表明糖尿病骨疾病的治疗潜力.
研究的目的:
- 开发一个体外模型来研究机械应变对肌分泌和骨代谢的影响.
- 研究不同运动方案 (END与HIIT) 在高血糖条件下对肌肉和骨结构的影响.
- 为了阐明肌肉和骨之间的生化交谈,脱离物理刺激.
主要方法:
- 在体外开发的骨结构使用交叉连接的等来进行骨质分化.
- 从纤维素创建肌肉结构,用于肌细胞分化和肌管形成.
- 在高血糖条件下,在耐力 (END) 和高强度间歇训练 (HIIT) 应变方案下,使用单种和联合培养与骨结构的骨基因表达进行了研究.
主要成果:
- 在单种植中,END和HIIT疗法都增加了INTERLEUQIN-15 (Il15) 和胰岛素类生长因子1 (Igf1) 的表达;END促进了更多的肌细胞分化.
- 与END相比,HIIT疗法在共同培养的肌肉结构中增强了葡萄糖运输体4 (Glut4) 表达和葡萄糖吸收.
- 在HIIT下肌肉构造促进了比END更健康,更成熟的骨表型,而在共同培养的肌肉中,肌态素 (Mstn) 表达被降低了.
结论:
- 开发的体外共同培养系统允许对机械应变进行直角操纵,并研究肌肉骨生化交叉交谈.
- 在这个模型中,HIIT疗法在葡萄糖吸收和骨健康上表现出优异的效果,与END相比.
- 这个系统可以帮助识别分子标,并开发用于代谢性骨病的工程疗法.
相关概念视频
01:22Muscle Stimulation Frequency
2.2K
The contraction strength of muscles is regulated by motor neurons, which modulate the frequency of action potentials dispatched to the motor units based on the body's requirements. This process of varying the muscle stimulation frequency allows muscles to contract with a force that is precisely tailored to the needs of the moment, whether lifting a feather or a heavy box.
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
2.2K
01:17Hormones and Bone Tissue
2.7K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
2.7K
01:13Formation of Muscle Fibers from Myoblasts
4.9K
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
4.9K