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在脑内出血中,细胞外矩阵分子的显著升高

Hongmin Li^1,2,3,4, Samira Ghorbani^3,4, Ruiyi Zhang^1,2,3,4

¹Department of Cerebrovascular Diseases, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

Frontiers in molecular neuroscience

|November 29, 2023

概括

脑内出血 (ICH) 改变了细胞外基质 (ECM) 分子,神经细胞可以选择性地增加. 这种分子阻碍了寡细胞的修复,可能会阻碍中风后的恢复.

科学领域:

神经科学是一个神经科学.
脑卒中研究脑卒中研究
细胞外矩阵生物学细胞外矩阵生物学

背景情况:

脑内出血 (ICH) 是一种严重的中风类型,死亡率高.
细胞外基质 (ECM) 分子沉积通常会阻碍再生,并在神经疾病中增强神经炎症.
在ICH发生后,ECM分子的特定变化和修复抑制作用在很大程度上是未知的.

研究的目的:

为了调查ICH后的ECM分子组成的变化.
为了确定特定的ECM成员,可能会抑制出血性中风后的修复.
探索已识别的ECM分子对神经修复过程的功能影响.

主要方法:

使用原酶诱导的ICH小鼠模型和人类ICH标本.
使用免疫光显微镜和共聚焦成像与3D染检查了ECM成员表达.
评估了ECM分子对组织培养中的寡类细胞前体细胞 (OPC) 的影响.

主要成果:

神经,一种冠状素硫酸蛋白甘 (CSPG),在小鼠和人类ICH中,在周血和病变核心组织中显著上调.
在ICH后观察到纤维素素,纤维素和肝素硫酸蛋白糖 (HSPG) 的水平升高.
纯化神经抑制了OPC粘附和工艺外生长在体外,对于髓化至关重要.

结论:

ICH诱导出明显的ECM变化,特别是神经瘤的选择性上调.
神经对寡头细胞系细胞产生负面影响,这表明它在阻碍修复方面发挥了作用.
在ICH后的ECM变化可能会对功能恢复和修复结果产生不利影响.

关键词:

顺德罗因丁硫酸盐蛋白质甘氨酸蛋白质甘氨酸细胞外矩阵是细胞外矩阵.脑内出血脑内出血神经炎症是一种神经炎症.复黄化是复黄化的一种.

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