数据挖掘揭示了Apis mellifera丝状病毒的组织特异表达和宿主血统相关的形式

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Robert S Cornman¹

¹U.S. Geological Survey, Fort Collins, Colorado, United States.

|November 29, 2023

概括

蜜蜂丝状病毒 (AmFV) 呈现出三种不同的血统,其地理分布与蜜蜂祖先有关. 病毒的表达因组织而异,在脑组织中检测到的水平较低.

科学领域:

病毒学病毒学
基因组学就是基因组学.
养蜂养蜂养蜂养蜂养蜂养蜂养蜂养蜂

背景情况:

蜜蜂丝状病毒 (Apis mellifera filamentous virus,AmFV) 是一种双链DNA病毒,感染蜜蜂的基因位置不明.
关于AmFV进化和感染机制的知识有限,由于同源性较弱,由于开放式读取框架 (ORF) 标注的挑战而复杂化.

研究的目的:

评估AmFV ORF,包括编码框架保护,编码偏差和净化选择.
量化AmFV内的遗传变异,并确定与感染率相关的宿主因素.
为了检查不同蜜蜂组织的病毒表达模式.

主要方法:

使用了来自NCBI序列读取档案 (SRA) 的短读序列数据.
扫描后的序列读取了AmFV特定的kmers,并对高数样本进行了映射和de novo组装.

主要成果:

确定了三种不同的AmFV进化系 (clades),其地理分布与蜜蜂祖先和感染水平相关.
标注了155个高可信度ORF,显示出高蛋白质相同性,低进化速率和中度至强度的编码子偏差,但没有强有力的证据表明积极选择.
在大部分基因组中检测到病毒RNA,具有复杂的转录结构和组织特异性表达,在脑组织中明显低于腹部组织.

结论:

这项研究揭示了AmFV及其蜜蜂宿主系之间的转录复杂性和共同进化关系.
这些发现有助于更好地了解这个神秘的病毒的进化和感染生物学.

关键词:

蜜蜂鸟 (Apis mellifera) 是一个有趣的动物.有丝状病毒Apis mellifera丝状病毒病毒的DNA 病毒的DNA.主体-病原体共同进化克默尔的分析

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