细胞质错折蛋白质聚合物的溶解中的固态到液态相位过渡
Alexis Tomaszewski1,2, Rebecca Wang3, Eduardo Sandoval4
1Center for Cell Dynamics and Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
iScience
|November 29, 2023
概括
细胞蛋白聚合物在热应力后溶解时从固态过渡到液态. 像Hsp104这样的伴侣蛋白对于这个过程和聚合物清除至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生物化学
- 蛋白质平衡是蛋白质的平衡.
背景情况:
- 蛋白质聚合物的积累与细胞衰老和神经退行性疾病有关.
- 蛋白质聚合物溶解的机制,特别是在压力下,尚未完全理解.
- 错误折叠的蛋白质的清除受损导致细胞功能障碍.
研究的目的:
- 使用活细胞成像研究酵母中蛋白质分解的动态过程.
- 阐明伴侣蛋白在压力诱导的蛋白质聚合物的溶解中的作用.
- 了解细胞恢复期间蛋白质聚合物的物理状态过渡.
主要方法:
- 使用定量活细胞成像技术来监测酵母中的蛋白质聚合物.
- 应用热应力来诱导蛋白质聚合,然后进行应力减弱.
- 评估了特定的伴侣蛋白 (Hsp104,Sse1) 在聚合动态中的作用.
主要成果:
- 热应力诱导的蛋白质聚合物存在于固体凝结物中.
- 在消除应力后,聚合物过渡到类似液体的状态,促进融合和溶解.
- Hsp104 (一个Clp/HSP100伴奏) 和Sse1 (一个HSP110伴奏) 对于这种固态到液态相转换 (SLPT) 和随后的聚合物清除至关重要.
结论:
- 蛋白质分解涉及到聚合物的意想不到的固态到液态相转换 (SLPT).
- 陪伴蛋白在调节总体物理状态和促进清除方面发挥着至关重要的作用.
- 这项研究揭示了细胞控制蛋白质分解的新机制,为衰老和疾病提供了洞察力.
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