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对基于人体外围血液单核细胞的体外疫苗评估系统的介质进行比较

Shuran Gong¹, Putri Fajar¹, Jacqueline De Vries-Idema¹

¹Department of Medical Microbiology & Infection Prevention, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.

Clinical and experimental vaccine research

|November 29, 2023

概括

罗斯韦尔公园纪念研究所 (RPMI) 介质最好支持人类外周血液单核细胞 (PBMC) 免疫反应,用于疫苗开发. 在维持细胞活力和免疫功能方面,RPMI优于Dulbecco的最小基本介质 (DMEM) 和Iscove的修饰DMEM (IMDM).

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
疫苗学疫苗学疫苗学

背景情况:

人类外周血液单核细胞 (PBMC) 对于体外疫苗开发和评估至关重要.
最佳的细胞性能需要适当的培养基来支持各种免疫细胞类型.

研究的目的:

为了比较罗斯韦尔公园纪念研究所 (RPMI),杜尔贝科的最小必需介质 (DMEM) 和伊斯科夫的修改DMEM (IMDM) 支持PBMC免疫反应.
评估不同介质支持托尔类受体 (TLR) 连接体和整个非活性病毒 (WIV) 流感疫苗引起的先天性和适应性免疫力的能力.

主要方法:

人类PBMCs在RPMI,DMEM或IMDM中培养,10%的胎儿小牛血清.
随着时间的推移,监测了细胞活力.
通过基因表达和蛋白质分泌来评估对TLR配体和WIV流感疫苗的免疫反应.

主要成果:

在RPMI和IMDM中,PBMC活力略高于在延长化期间的DMEM.
RPMI对先天免疫基因表达 (MyD88,MxA,ISG56,MCP1) 和抗原呈现细胞激活表现出优越的支持.
与DMEM相比,RPMI和IMDM在WIV刺激时支持更高数量的IFNγ产生T细胞和更大的免疫球蛋白G分泌.

结论:

罗斯韦尔公园纪念研究所 (RPMI) 介质被确定为基于人类PBMC的体外疫苗评估系统的最佳选择.
RPMI有效地支持先天性和适应性免疫反应,这对于疫苗评估至关重要.

关键词:

文化媒体文化在体外 (in vitro) 的情况下.接种流感疫苗可以预防流感有关联体的连接体周围血液中的单核细胞.收费类受体的收费类受体

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