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类似T5的菌体BF23避免了宿主介导的DNA限制和甲基化

Mikhail Skutel¹, Aleksandr Andriianov¹, Maria Zavialova^1,2

¹Skolkovo Institute of Science and Technology, Bolshoy Boulevard 30/1, 143028, Moscow, Russia.

|November 29, 2023

概括

菌体BF23,菌体T5的亲属,已经对其全基因组进行了测序. 这项研究揭示了BF23如何抵抗宿主免疫系统并避免甲基化,为Tequintavirus菌体生物学提供了洞察力.

科学领域:

病毒学病毒学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

菌体BF23是一种Tequintavirus,与菌体T5密切相关,已知可以感染大肠杆菌.
BF23与T5具有共同的特征,包括长端重复,两阶段的基因组注入过程和特定的DNA缺口.
之前的研究只确定了BF23的部分基因组片段,特别是编码受体相互作用蛋白质的片段.

研究的目的:

确定菌体BF23.3的完整基因组序列.
为了描述BF23病毒的蛋白质组成.
调查BF23对宿主免疫系统使用的反限制机制.

主要方法:

细菌的全基因组测序 BF23.3.
对BF23病毒的蛋白质组分析.
实验测试BF23对I型,II型和III型限制修改系统的抗性.
研究BF23与宿主甲基化过程的相互作用.

主要成果:

细菌体BF23的完整基因组序列得到了阐明.
BF23利用BtuB受体进入宿主细胞,与T5的FhuA.不同.
BF23表现出对I,II和III类限制修改系统的抗性,特别是当识别站点位于终端重复之外时.
BF23积极避免宿主介导的DNA甲基化.

结论:

BF23的完整基因组序列提供了对这种类似T5的菌体的全面了解.
BF23采用特定的策略来规避宿主限制-修饰系统和甲基化.
甲基化抑制可能是Tequintavirus和Epseptimavirus菌体中保存的特征,尽管与它们的抗限制能力没有直接联系.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.埃舍里希亚菌体T5菌体反限制措施的限制.细菌菌体是一种菌体.基因组基因组是基因组的组成部分.甲基化处理的方法菌体 BF23 的情况.蛋白质组是一种蛋白质组.

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