在智利的猪群中识别和描述猪类罗塔病毒A
Victor Neira1, Cristián Melgarejo1, Constanza Urzúa-Encina1
1Departamento de Medicina Preventiva Animal, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago, Chile.
Frontiers in veterinary science
|November 29, 2023
概括
智利猪中的罗塔病毒A (RVA) 显示了潜在的动物传播. 全基因组测序确定了与全球人类和猪变种密切相关的RVA菌株,强调需要持续监测.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 公共卫生 微生物学
背景情况:
- 罗塔病毒A (RVA) 在新生猪中引起严重的腹和经济损失.
- 由于其遗传进化,流行率和人类和动物的病原性,RVA引起了公众健康的关注.
研究的目的:
- 在智利的养猪场中识别和表征罗塔病毒A菌株.
- 调查智利猪群中RVA的遗传多样性和潜在的动物源源.
主要方法:
- 从22个养猪场收集了154个口腔液体和便样本.
- 使用实时RT-PCR检测RVA.
- 基因分析的阳性样本和分离物的全基因组测序.
主要成果:
- 来自14个农场的58%的样本呈RVA阳性.
- 整个基因组测序揭示了G5/G3-P[7]-I5-R1-C1-M1-A8-N1-T1-E1-H1.1的基因组星座.
- 遗传学分析表明,智利RVA菌株的起源是混合的,与全球人类和猪RVA有密切的关系.
结论:
- 智利猪RVA菌株表现出与全球发现的人类和猪RVA密切相关的遗传特征.
- 这些发现凸显了在智利猪场中流传的RVA的潜在动物传染性.
- 持续的全球RVA全基因组测序对于理解流行病学和检测动物感染菌株至关重要.
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