解锁微藻宿主 - - 探索暗生长的微藻的转化,以实现可持续的高价值植物化学品生产
Surumpa Jareonsin1,2, Kanjana Mahanil1, Kittiya Phinyo1,3
1Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
Frontiers in bioengineering and biotechnology
|November 29, 2023
概括
这项研究介绍了一种新的暗宿主微藻平台,使用Chlorella sorokiniana进行高效的基因工程. 这种可持续的方法有助于在没有轻便或昂贵的设备的情况下生产高价值化合物.
科学领域:
- 生物技术和合成生物学
- 微藻工程 微藻工程
- 可持续的生物生产
背景情况:
- 微藻为生物生产提供了与陆地植物相比的可持续优势.
- 微藻用于基因表达的异质培养 (暗模式) 尚未得到充分研究.
- 科洛雷拉索罗基尼亚纳 (Chlorella sorokiniana) 呈现出快速增长,多样化的碳来源利用和废水处理潜力.
研究的目的:
- 推进微藻基因工程,用于暗种植.
- 确定Chlorella sorokiniana作为异质基因表达的强大的暗宿主.
- 为了使高价值植物化学品和食品和料的成分的生产.
主要方法:
- 在异构条件下对11种抗生素进行有效可选择标记的选.
- 通过Agrobacterium共同培养在完全黑暗中的Chlorella sorokiniana的转化.
- 使用绿色光蛋白 (GFP) 和β-葡萄糖酶 (GUS) 表达的成功转化验证.
主要成果:
- 鉴定出高增长抑制率 (98%,93%,92%),高效选择性标记剂的G418,菌素和菌素.
- 在黑暗条件下,在Chlorella sorokiniana中成功实现异质基因转换.
- 使用微藻作为黑暗宿主来产生有价值的化合物的可行性.
结论:
- 开创了一个使用Chlorella进行异质基因转换的暗宿主微藻平台.
- 开发了微藻基因工程的可访问协议,消除了对轻便和昂贵设备的需求.
- 建立了一种可持续的方法,使用异质微藻生产高价值的营养素,补充剂和药品.
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