石榴皮提取物对卵巢细胞过程的关键调节者的调节作用in vitro
Adriana Kolesarova1,2, Simona Baldovska1, Ladislav Kohut1
1Institute of Applied Biology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovakia.
Frontiers in endocrinology
|November 29, 2023
概括
石榴皮提取物 (PPE) 对卵巢细胞具有差异性作用,在粒粉细胞中起到抗氧化作用,在癌细胞中起到抗氧化作用. 这表明了卵巢癌预防和管理的潜力.
科学领域:
- 生物化学和分子生物学
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 卵巢细胞,包括粒素 (HGL5) 和腺癌 (OVCAR-3) 线,研究了它们对石榴皮提取物 (PPE) 的反应.
- 聚化合物,特别是基,被确定为PPE的主要成分,具有显著的抗氧化活性.
研究的目的:
- 评估短时间治疗红皮提取物 (PPE) 对人类卵巢细胞系 (HGL5和OVCAR-3) 的体外影响.
- 研究PPE的作用机制,包括其对细胞活力,增殖,活性氧物种 (ROS),生长因子信号和类固醇生成的影响.
主要方法:
- 人类颗粒素 (HGL5) 和卵巢腺癌 (OVCAR-3) 细胞的细胞培养.
- 用石榴皮提取物 (PPE) 治疗24小时.
- 分析多含量,总含量 (TPC),抗氧化活性,代谢活性,循环素依赖激酶1 (CDKN1A) 水平,细胞活力 (流细胞计),ROS生成,生长因子和受体表达 (TGF-β2,EGF,TGFBR2,EGFR) 和类固醇生成 (17β-雌激醇分泌).
主要成果:
- 在较高剂量时,PPE对HGL5细胞表现出抗氧化作用,对OVCAR-3细胞表现出抗氧化作用,从而增加ROS生成.
- 在OVCAR-3细胞中,PPE抑制了代谢活动并增加了CDKN1A水平,同时降低了TGF-β2,EGF,TGFBR2和EGFR.
- 在HGL5细胞中,PPE刺激了17β-雌激醇的分泌,这表明它对类固醇生成有影响.
结论:
- 石榴皮提取物含有对卵巢细胞具有差异作用的生物活性化合物,表明在卵巢生理学中起着复杂的作用.
- PPE独特的抗氧化和抗氧化活性,以及其对生长因子和类固醇生成的调节,突出了其在卵巢癌 (化疗) 预防和管理策略中的潜力.
关键词:
HGL5 HGL5 HGL5 HGL5 HGL5 HGL5 HGL5 HGL5 HGL5 HGL5在OVCAR-3中使用OVCAR-3.普尼卡·加拉纳图姆 (Punica garanatum L.) 的一种.灭症 (apoptosis) 是一种死亡的过程.增长因素 增长因素 增长因素它们的扩散和扩散.有活性氧物种的反应性氧物种.类固醇的产生.更多相关视频
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