长非编码RNAs的全基因组识别及其在卜对盐应激反应中的潜在功能
Xiaochuan Sun1, Mingjia Tang2, Liang Xu3
1School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian, China.
Frontiers in genetics
|November 29, 2023
概括
这项研究确定了卜中的新型长非编码RNAs (lncRNAs),这些RNAs对盐应激有反应. 这些lncRNAs调节了参与植物防御的基因,为卜盐耐受机制提供了洞察力.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 无生物应激反应应激反应
背景情况:
- 长非编码RNAs (lncRNAs) 是植物基因表达的关键调节者,特别是在压力下.
- 高盐度的土壤严重影响作物产量,卜是适度敏感的.
- 在卜盐耐受性中 lncRNAs 的作用尚不清楚.
研究的目的:
- 为了进行基因组范围的识别和分析盐反应的lncRNAs在卜.
- 预测这些差异表达的 lncRNA 的目标基因.
- 阐明lncRNAs在卜对盐度的反应中的调节作用.
主要方法:
- 在盐度处理下,卜肉根的链特异性RNA测序.
- 新型 lncRNAs 的全基因组识别.
- 对差异表达的 lncRNAs 的 cis 和 trans 作用的目标基因的预测.
主要成果:
- 鉴定了7,709个新的lncRNAs,其中363个在盐应激下显著差异地表达.
- 预测了5,006个cis基因和5,983个trans基因对这些对盐有反应的lncRNAs.
- 目标基因参与关键的防御途径:信号转导,离子稳态,透调节和光合作用.
结论:
- 这是第一个关于卜中对盐有反应的lncRNAs的全基因组综合性研究.
- 已识别的lncRNA及其点为了解卜盐应激防御机制提供了基础.
- 未来的功能研究可以利用这些发现来改善卜的盐分耐受性.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
02:02Responses to Salt Stress
13.1K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.1K
01:23Types of RNA
63.8K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.8K
02:24Ribosome Profiling
3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K