对患有或没有肺癌的患者的唾液外体miRNA进行比较分析
Mengfeng Liu1, Xiran Yu1, Jianlong Bu1
1Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
Frontiers in genetics
|November 29, 2023
概括
唾液外基因组含有微RNA (miRNA),在肺癌 (LC) 患者和非癌症患者之间存在差异. 这些特定的miRNA显示出作为早期肺癌检测的新生物标记物的潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 肺癌是全球癌症死亡的主要原因之一.
- 外体,细胞释放的小囊泡,在癌症进展和细胞间通信中发挥作用.
- 唾液外基因组正在成为非侵入性癌症生物标志物发现的潜在来源.
研究的目的:
- 研究唾液外体微RNAs (miRNAs) 作为肺癌 (LC) 的非侵入性生物标志物的潜力.
- 与非癌症对照组 (NC) 相比,从LC患者的外体中识别差异表达的miRNAs (DE miRNAs).
主要方法:
- 从LC和NC个体收集唾液样本.
- 通过超离心法从唾液中分离出外体.
- 在外体RNA上进行了MiRNA测序,随后使用dgeR进行了差异表达分析.
- 定量实时聚合酶连锁反应 (qPCR) 用于验证选定的DE miRNAs.
主要成果:
- 总共有372个miRNA在唾液外体中被鉴定出来.
- 在比较LC与NC组时,发现了15个DE小RNA (8个上调,7个下调).
- 对高调节的DE小RNA目标基因的功能分析显示了癌症相关途径的丰富.
- 使用qPCR成功验证了三种DE小RNA.
结论:
- 唾液外体内的miRNAs在肺癌患者中表现出差异化的表达模式.
- 这些唾液外体miRNAs有望成为预测和诊断肺癌的潜在生物标志物.
- 通过分析唾液外体miRNAs,非侵入性检测肺癌可能是可行的.
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