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DASES:用于食道状细胞癌的替代拼接的数据库

Yilong Chen^1,2, Yalan Kuang^1,2, Siyuan Luan¹

¹Department of Thoracic Surgery and West China Biomedical Big Data Center, West China Hospital, Sichuan University, Chengdu, China.

Frontiers in genetics

|November 29, 2023

概括

一个新的数据库,DASES,在食道状细胞癌 (ESCC) 中识别了新的替代拼接事件 (ASEs) 和长非编码RNA (lncRNAs). 本资源有助于理解ESCC分子机制,并有助于研究.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

食道癌是全球癌症死亡的主要原因,特别是亚洲的食道状细胞癌 (ESCC).
替代拼接 (AS) 显著影响ESCC的发展,但对于ESCC特定的AS事件缺乏专门的数据库.
现有的数据库往往忽略了长非编码RNAs (lncRNAs) 在ESCC分子机制中的作用.

研究的目的:

开发一个专门的数据库,DASES,用于食道状细胞癌 (ESCC) 的替代拼接事件 (ASEs).
在一大批ESCC样本中全面分析lncRNA和基于mRNA的ASE.
为了解ESCC分子病理学提供一个有价值的资源.

主要方法:

利用来自346个ESCC样本的公开可用和内部RNA测序数据集 (93%的瘤/非瘤配对).
开发和部署了DASES数据库 (http://www.hxdsjzx.cn/DASES) 用于分析替代拼接事件.
在瘤和相邻的正常组织之间进行对对比,以确定ESCC相关的ASE.

主要成果:

在食道状细胞癌中鉴定了257种新型长非编码RNA (lncRNAs).
发现了59,094个可能与ESCC相关的替代拼接事件 (ASEs).
DASES集成了用于ESCC的lncRNA和mRNA拼接数据.

结论:

通过提供对ESCC替代拼接活动的全面洞察力,DASES解决了关键的差距.
数据库有助于更深入地了解ESCC背后的分子机制.
DASES是ESCC研究界的一个有价值的资源.

关键词:

替代性拼接是一种替代性的拼接.我们的数据库数据库数据库数据库.食管状细胞癌的癌症这种异形异形是什么?新的 lncRNA 是一个新的 lncRNA.

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