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在的花过渡期间的差异基因表达

Robert E Paull¹, Najla Ksouri², Michael Kantar¹

¹Tropical Plant & Soil Sciences University of Hawaii at Manoa Honolulu Hawaii USA.

|November 29, 2023

概括

乙烯处理通过改变基因表达来诱导的开花,促进从植物生长到花发展的过渡. 像APETALA1/FRUITFULL (AP1/FUL) 和FLOWERING LOCUS T (FT) 这样的关键基因对于这个过程至关重要.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

和装饰花的商业开花是使用乙烯或其类似物诱导的.
乙烯处理触发了植物顶部从植物性向花性水系的转变.
在810天内观察到形态变化,花开发生在810周后.

研究的目的:

研究中乙烯诱导的开花背后的分子机制.
为了确定差异表达基因 (DEGs) 和关键的调节途径,涉及到花过渡.
开发一种花开花时的乙烯反应模型.

主要方法:

在8个采样阶段 (治疗后6小时至8天) 进行基因表达分析.
在乙烯应用后对差异性基因表达 (DE) 的分析.
预测乙烯反应转录因子 (ERTF) 的目标基因.

主要成果:

在乙烯处理后,超过7961个基因表现出差异性表达.
三个ERTF被上调,准了像CONSTANS-like 3 (CO),WUSCHEL,APETALA1/FRUITFULL (AP1/FUL) 和莉酸合成基因这样的基因.
包括开花点T (FT),终端花 (TFL),类似AGAMOUS的APETELAR (AP2) 和SEPETALA (SEP) 在内的基因在2~3天内迅速增加.

结论:

乙烯通过AP1/FUL直接促进的植物向花的过渡,与SPL,FT,TFL,SEP和AP2相互作用.
已经失去了开花抑制器开花 LOCUS C.
已识别的候选基因为基因操纵提供了目标,以了解它们在花过渡中的作用.

关键词:

这就是AGAMOUS.在APETALA1/FRUITFULL (AP1/FUL) 中使用.开花的位置T T 在Trans-Cis图案中.乙烯反应转录因子的乙烯反应转录因子.花的过渡期花的过渡期花的监管者是花的监管者

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