使用全基因组关联研究来剖析大米 (Oryza sativa L.) 中的基因组区域和潜在的围病耐药性特征
R Naveenkumar1,2,3, Annamalai Anandan1,4, Seenichamy Rathinam Prabhukarthikeyan5
1Crop Improvement Division ICAR-National Rice Research Institute (NRRI) Cuttack Odisha India.
Plant direct
|November 29, 2023
概括
研究人员使用全基因组关联研究 (GWAS) 确定了新来源的米虫害耐药性. 这些发现将有助于开发耐用的耐ShB米品种.
科学领域:
- 植物病理学 植物病理学
- 遗传学 遗传学 是一个
- 农业学是一种农业学.
背景情况:
- 米的生产力受到 *Rhizoctonia solani * 的显著影响,这种真菌会引起囊病 (ShB).
- 有限的米种群对ShB具有耐药性,阻碍了育种工作.
- 识别ShB耐药源及其遗传基础对于全球粮食安全至关重要.
研究的目的:
- 识别米中ShB耐药性的新型来源.
- 了解ShB抗性的遗传性和特征相互作用.
- 通过全基因组关联研究 (GWAS) 精确确定与ShB耐药性相关的基因组区域.
主要方法:
- 对ShB抗性评估了330个大米加入,为GWAS创建了192个核心小组.
- 使用百分比疾病指数 (PDI) 和疾病进展曲线下的区域 (AUDPC) 计算了ShB感染率.
- 用133个SSR标记器对面板进行基因定型,并使用GLM和MLM (Q+K) 模型来识别标记器-特征关联 (MTA).
主要成果:
- 观察到ShB耐药性和农业形态特征的显著表型变异.
- 确定了30个MTA,其中8个是在染色体2,4,8,10和12上的新的定量特征位置 (QTL),解释了5-15%的表型变异.
- 包括Os05t0566400,Os08t0155900和Os09t0567300在内的6个候选基因被确定为ShB防御机制.
结论:
- 确定了新的ShB耐药性捐赠者:IC283139,IC 277248,Sivappuchithirai Kar和Bowalia. 这两种新型的捐赠者.
- 12个染色体中的10个染色体上的基因组区域与ShB耐药性有关.
- 这些发现为开发具有耐久性抗ShB米品种提供了宝贵的遗传资源.
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