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使用隔间自我复制和微流体平台解开Phi29 DNA聚合酶的盐分耐受性

Yaping Sun1, Danny Hsu Ko2, Jie Gao2

  • 1Research Center of Molecular Diagnostics and Sequencing, Research Institute of Tsinghua University in Shenzhen, Shenzhen, China.

Frontiers in microbiology
|November 29, 2023
PubMed
概括

为高盐条件优化phi29聚合酶可以增强纳米孔测序. 突变提高了盐分耐受性和过程性,这对于实时测序应用至关重要.

关键词:
分隔式自我复制 (CSR)保守的氨基酸.基于滴滴的微流体学盐耐受性聚合酶是一种盐耐受性聚合酶.通过合成 (SBS) 进行测序.

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