pH值和介质成分对长链与产生C4C8脂肪酸的Megasphaera hexanoica的链延长的影响
Carla Fernández-Blanco1, María C Veiga1, Christian Kennes1
1Chemical Engineering Laboratory, Faculty of Sciences and Interdisciplinary Centre of Chemistry and Biology - Centro Interdisciplinar de Química y Biología (CICA), BIOENGIN Group, University of A Coruña, Coruña, Spain.
Frontiers in microbiology
|November 29, 2023
概括
这项研究优化了使用Megasphaera hexanoica的链延长,通过调整介质和pH值来实现更高的价值中链脂肪酸 (MCFA) 产量. 结果显示了增强MCFA生物生产的潜力.
科学领域:
- 生物技术和工业微生物学
- 无氧发酵是一种无氧发酵.
- 代谢工程是代谢工程.
背景情况:
- 链延长技术将短链基质转化为有价值的长链产品,如中链脂肪酸 (MCFA).
- 虽然Clostridium kluyveri已被广泛研究,但其他有前途的链延长物,如Megasphaera hexanoica需要进一步调查.
- 优化M. hexanoica的条件可以释放其有效生产MCFA的潜力.
研究的目的:
- 为了确定Megasphaera hexanoica的最佳生长和发酵条件.
- 为了评估使用M. hexanoica. 的中链脂肪酸 (MCFA) 的产量.
- 评估不同介质成分和pH值对M. hexanoica表现的影响.
主要方法:
- 用两种不同的介质配方进行了批量瓶研究,以评估M. hexanoica的生长.
- 在不同的pH值 (5.8,6.5,7.2) 进行了批量生物反应器实验,以研究乳酸盐和酸盐发酵.
- 在进一步的生物反应器实验中操纵了基质度,以调查它们对MCFA生产的影响.
主要成果:
- 含有酵母提取物的介质显示出有利的生产/成本比率,产生2.62g/L的n-caproate.
- 生物反应器研究证实,在测试的pH值中,C4-C8脂肪酸的产生一致.
- 在pH 6.5的乳酸盐和酸盐度翻倍显著提高了MCFA的产量,达到3.7g/L的n-caproate和1.5g/L的n-caprylate,增加了H2和CO2的产生.
结论:
- 基于酵母提取物的介质和特定的pH条件对于优化M. hexanoica性能至关重要.
- 黑色菌显示出从乳酸和酸中产生有价值的MCFA的巨大潜力.
- 进一步优化基质度和发酵参数可以提高MCFA的生产力.
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