多塞塔克塞尔抑制了人类乳腺细胞中的上皮-介质细胞过渡
Samuel J Beerkens1, Jessica J King1, Kelly L Irving1
1School of Molecular Sciences, The University of Western Australia, 35 Stirling Highway, Perth, Western Australia 6009, Australia.
Molecular pharmaceutics
|November 29, 2023
概括
低度的多塞塔克塞尔抑制了表皮细胞-介质细胞过渡 (EMT),这是一个与癌症扩散和耐药性相关的过程. 这表明多塞塔克塞尔可以用于阻止转移并提高化疗的有效性.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 表皮-介质细胞过渡 (EMT) 增强癌细胞干细胞和迁移,促进转移.
- EMT与对化学疗法药物如多塞塔克塞尔的耐药性有关.
- 矛盾的是,目前的多塞塔塞尔治疗可以促进EMT,导致瘤复发.
研究的目的:
- 对人类乳腺上皮细胞中低致死性多塞塔克塞尔度对EMT的影响进行研究.
- 确定多塞塔克塞尔是否可以抑制EMT进展及其相关特征.
主要方法:
- 不朽化的人类乳腺上皮细胞 (HMLE) 用多塞塔克塞尔治疗IC50以下度.
- 免疫光和流动细胞计用于分析EMT标记物.
- 大量转录组测序评估了与EMT相关的全球基因表达变化.
主要成果:
- 长时间的多塞塔克塞尔治疗抑制了HMLE细胞中的EMT.
- 多塞塔克塞尔治疗导致上皮质标记物的上调.
- 多塞塔克塞尔治疗导致中酶体标志物的下调.
结论:
- 在特定度下,多塞塔克塞尔可以抑制EMT.
- 这种抑制作用表明了超出细胞毒性的潜在临床应用.
- 多西素可以作为一种EMT抑制剂来对抗转移和多药物耐药性.
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