三的力量:用于自然杀手 (NK) 细胞免疫工程的纳米材料,如果它们利用多受体刺激,就会最大限度地提高它们的效力
Helena Dodd1,2,3, Nadia Guerra2, Iain E Dunlop1
1Dept. Materials, Imperial College London, Exhibition Road, London, SW7 2AZ, UK.
Advanced healthcare materials
|November 29, 2023
概括
新的生物材料通过同时激活多个受体来激活自然杀手 (NK) 细胞. 这些纳米集群使用石墨烯氧化物板,显著增强NK细胞免疫疗法潜力.
科学领域:
- 生物材料科学 生物材料科学
- 免疫学 免疫学 免疫学
- 纳米技术 纳米技术
背景情况:
- 新兴的癌症免疫疗法侧重于自然杀手 (NK) 和T细胞激活.
- 目前的免疫工程纳米材料难以与分子试剂性能相匹配.
- 生理细胞激活涉及通过多个免疫受体进行协调信号传递,这是现有生物材料中缺乏的特征.
研究的目的:
- 开发能够同时刺激多个NK细胞免疫受体的新生物材料.
- 为了工程纳米材料,提高NK细胞激活的效力,以改善癌症免疫疗法.
- 研究纳米聚类和多受体刺激对NK细胞反应的协同效应.
主要方法:
- 使用纳米级石墨烯氧化物 (NGO) 片 (≈75 nm) 作为模板来创建单克隆抗体 (mAb) 的纳米集群.
- 研究了一种带有固定mAb的平面基质模型系统,以告知纳米剂设计.
- 开发并测试了基于非政府组织的纳米集群,结合mAbs,针对三个特定的NK细胞激活受体:NKP46,NKG2D和DNAM-1.
主要成果:
- 由三个mAbs组合激活的NK细胞显示出比单个mAbs或对激发的NK细胞更大的效力.
- 设计的NGO-mAb ((αNKP46 + αNKG2D + αDNAM-1) 纳米集群显著优于单受体纳米集群.
- 与平面基质相比,NGO-mAb纳米激活的NK细胞数量几乎是NGO-mAb ((αNKP46) 的两倍,并且在相当的mAb剂量下表现出比平面基质更高的功效.
结论:
- 在非政府组织的表格上对抗体进行纳米聚类是免疫工程生物材料的一个有希望的策略.
- 通过工程纳米集群同时刺激多个NK细胞受体,可以增强NK细胞的激活.
- 这种方法为设计用于增强免疫治疗的先进生物材料提供了一个新的范式.
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