通过线下SESI-HRMS进行分析的呼气样本的保存:概念验证研究
Rosa A Sola-Martínez1, Jiafa Zeng2,3, Mo Awchi2,3
1Department of Biochemistry and Molecular Biology B and Immunology, University of Murcia, Murcia, Spain.
Journal of breath research
|November 29, 2023
概括
使用二次电喷射电离高分辨率质谱仪 (SESI-HRMS) 进行远程呼吸分析是可行的. 在Nalophan®袋中呼出的呼吸样本可以在化合物强度的最小损失下存储数小时,从而使灵活的离线临床研究成为可能.
科学领域:
- 分析化学 分析化学
- 生物医学科学 生物医学科学
- 环境健康 环境健康
背景情况:
- 二次电喷射电离高分辨率质谱 (SESI-HRMS) 提供快速,灵敏和选择性的呼吸分析.
- 目前的SESI-HRMS应用程序需要在分析站点实时测量,限制了临床可访问性.
- 在Nalophan®袋中进行远程呼吸采样,为离线SESI-HRMS分析提供了一个潜在的解决方案.
研究的目的:
- 评估储存时间和温度对储存在纳洛®袋中的呼气样本的影响.
- 在不同存储条件下,评估27种与氧化应激相关的化物的稳定性.
- 确定离线SESI-HRMS分析对临床应用的可行性.
主要方法:
- 在Nalophan®袋中收集了呼出的呼吸样本.
- 样品在室温和干冰上存储时间不同.
- 使用SESI-HRMS测量化合物强度,包括27种特定的化物,并与未储存的样本进行比较.
主要成果:
- 经过2个小时的储存,在两个测试温度下,平均信号强度保持在80%以上.
- 对于27个化物,储存24小时后的平均相对强度损失低于20%.
- 与干冰相比,在室温下储存的样品中,化物强度通常更高,这可能是由于水的凝结.
结论:
- 呼出的呼吸样本可以在Nalophan®袋中存储几个小时,分析信号的损失最小.
- 这种方法允许在离线SESI-HRMS研究中提供灵活的物流,扩大其临床实用性.
- 阿尔代的稳定性表明,可以远程监测氧化应激生物标志物.
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