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骨髓干细胞衍生的β细胞:糖尿病细胞治疗的新问题

Hadeer A Aglan¹, Soheir E Kotob², Nadia S Mahmoud¹

¹Hormones Department, Medical Research and Clinical Studies Institute, National Research Centre, Dokki, Giza, Egypt; Stem Cell Lab., Center of Excellence for Advanced Sciences, National Research Centre, Dokki, Giza, Egypt.

|November 29, 2023

概括

骨髓介质干细胞 (BM-MSCs) 可以分化为产生胰岛素的细胞 (IPCs),以治疗高血糖症. 这种细胞疗法对开发一种治疗糖尿病的新疗法充满希望.

科学领域:

干细胞生物学干细胞生物学
内分泌学在内分泌学.
再生医学是一种再生医学.

背景情况:

高血糖是糖尿病 (DM) 的标志,需要新的治疗策略.
骨髓介质干细胞 (BM-MSCs) 提供了分化成各种细胞类型的潜力.
从干细胞生成功能性胰岛素生成细胞 (IPC) 是DM研究的一个关键目标.

研究的目的:

评估由骨髓-介质干细胞 (BM-MSCs) 衍生的胰岛素生成细胞 (IPCs) 在改善大鼠实验诱导的高血糖症方面的疗效.
确定最佳的差异化协议,从BM-MSCs生成功能IPC.
在糖尿病大鼠模型中评估移植的IPC的治疗潜力.

主要方法:

骨髓介质干细胞 (BM-MSCs) 用三个不同的协议分化为胰岛素生成细胞 (IPCs).
在体外评估包括基因表达分析 (Foxa-2,PDX-1,Ngn-3) 和胰岛素释放试验.
在体内研究中,将最具功能性IPC移植到糖尿病Wistar大鼠中,然后进行生物化学,分子和组织学分析.

主要成果:

差异化方案3产生了最具功能性的IPCs,具体基因标记物 (Foxa-2,PDX-1,Ngn-3) 的显著上调和强大的胰岛素分泌证明了这一点.
将这些IPC移植给糖尿病老鼠显著降低了血清葡萄糖,维斯法廷和胰腺葡萄糖水平.
移植IPC还导致血清胰岛素和C-水平增加,胰腺基因 (Foxa-2,Sox-17,IGF-1,FGF-10) 的表达增强,胰腺组织学得到改善.

结论:

骨髓介质干细胞 (BM-MSCs) 可以有效地分化为功能性胰岛素生成细胞 (IPCs),使用特定的协议 (协议3).
使用这些衍生IPC的细胞疗法在糖尿病大鼠模型中显示出在管理高血糖和恢复代谢平衡方面具有显著的潜力.
这种方法有望开发一种新的基于细胞的糖尿病治疗策略.

关键词:

糖尿病是一种糖尿病.产生胰岛素的细胞产生胰岛素.介质细胞干细胞介质细胞干细胞它们是老鼠,老鼠,老鼠.链条毒素 (Streptozotocin) 是一种毒素.

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