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DgHDA6通过提高ROS清理能力来增强花的耐寒性

Xingsu Zhang¹, Qinhan Zeng¹, Qing Yang¹

¹Department of Ornamental Horticulture, 211 Huimin Road, Chengdu 611130, China.

Ecotoxicology and environmental safety

|November 29, 2023

概括

过度表达DgHDA6基因增强了花的抗寒能力. 这涉及增加保护性化合物和抗氧化活性,改善低温压力下的生存能力.

科学领域:

植物科学植物科学
分子生物学分子生物学
压力生理学压力生理学

背景情况:

基因组脱乙酶 (HDACs) 对于植物对环境压力的反应至关重要.
在花的低温应激反应中,HDACs的具体机制尚不清楚.

研究的目的:

为了研究冷诱导的素脱乙酶基因DgHDA6在花 (Chrysanthemum morifolium Ramat) 中的作用.
为了确定DgHDA6是否增强香的寒冷耐受性.

主要方法:

对DgHDA6基因的分离和表征.
在莉花中过度表达DgHDA6.
通过测量存活率,生化指标 (proline,可溶性蛋白/糖,H2O2,O2-,MDA) 和低温压力下的抗氧化酶活动来评估耐寒性.
对寒冷应激反应基因表达的分析 (DgCuZnSOD,DgCAT,DgP5CS,DgFAD).

主要成果:

DgHDA6基因编码了一种具有典型脱乙化域的蛋白质.
过度表达DgHDA6显著改善了花在寒冷压力下生存率.
关键的保护性化合物和抗氧化酶活动增加,而氧化损伤标志物在DgHDA6过度表达线下降.
几种寒冷应激反应基因的表达因寒冷而上调.

结论:

DgHDA6在花对低温压力的反应中起着至关重要的作用.
过度表达DgHDA6通过改善反应性氧物种 (ROS) 的清理来增强耐寒性.

关键词:

抗氧化酶酶是一种抗氧化酶.希斯脱乙酶的使用情况低温应力压力低温应力压力奥斯莫斯调整的调整

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