在模拟的肠道微生物群上,通过六种工程饮食纳米颗粒 (EDN) 进行分泌体代谢失调的比较
Yijun Fan1, Tharushi Prabha Keerthisinghe2, Min Nian3
1Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Anhui Medical University, No 678 Furong Road, Hefei 230601, Anhui, China.
Journal of hazardous materials
|November 29, 2023
概括
工程食纳米颗粒 (EDN) 改变了肠道细菌的新陈代谢,影响了托和胆酸代谢等关键途径. 代谢分析揭示了不同的模式和敏感的生物标志物来预测这些相互作用.
科学领域:
- 环境健康 环境健康
- 微生物学 微生物学
- 纳米技术 纳米技术
背景情况:
- 工程食纳米颗粒 (EDN) 越来越多地用于食品中,引发了对潜在肠道微生物群暴露的担忧.
- 对于EDN对肠道细菌代谢的影响尚不清楚,因此需要对它们的相互作用进行研究.
研究的目的:
- 研究六种不同的EDN对模拟肠道微生物群的代谢活动的影响.
- 识别EDN暴露改变的特定代谢物和代谢途径,并建立剂量反应关系.
主要方法:
- 利用基于液态染色学和质谱学 (LC-MS) 的代谢学来分析模拟肠道微生物组的秘密组.
- 在两次饮食剂量中暴露了微生物组的六种EDN (Chitosan,CNC,CNF,PLGA,TiO2,SiO2).
主要成果:
- 所有六个EDN都改变了肠道微生物群的分泌组合,其中素,PLGA和SiO2显示出最显著的变化.
- 主要受影响的途径包括托,醇,胆酸和氨酸代谢.
- 氨酸和特定的胆酸 (氧化胆酸,胆酸) 被确定为EDN-肠道细菌相互作用的敏感生物标志物,表现出明显的剂量依赖反应.
结论:
- 代谢特征是敏感且可行的指标,用于理解和预测EDN与肠道微生物群之间的相互作用.
- 确定了可以作为这些相互作用的代表生物标记物的关键代谢物.
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