基于ployA-DNA/miRNA/AuNPs增强的探针三明治结构的SPRi/SERS双模式生物传感器,用于检测多个miRNA生物标志物
Yifan Li1, Li Jiang1, Zizhen Yu1
1College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018, China.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|November 29, 2023
概括
这项研究介绍了一种双模式生物传感器,用于使用microRNA (miRNA) 生物标志物进行敏感和可靠的早期癌症检测. 这种创新方法通过结合两个检测方法来提高准确性,从而改善早期癌症诊断.
科学领域:
- 生物医学工程 生物医学工程
- 分析化学 分析化学
- 在瘤学瘤学.
背景情况:
- 微RNAs (miRNAs) 是癌症早期检测的关键生物标志物.
- 现有的检测方法可能缺乏早期诊断所需的灵敏度或特异性.
- 需要高通量,可靠的方法来同时检测多个与癌症相关的miRNA.
研究的目的:
- 开发一种新的双模式生物传感器,将表面等离子体共振成像 (SPRi) 和表面增强拉曼光谱 (SERS) 结合起来,用于增强miRNA检测.
- 利用金纳米粒子 (AuNPs) 作为增强介质来提高传感器性能.
- 为了在血清中实现三种特定的与宫癌相关的miRNAs (miRNA21,miRNA124,miRNA143) 的高通量和灵敏检测.
主要方法:
- 制造与AuNPs集成的金芯片,用于双模式SPRi/SERS检测.
- 使用了三明治测试结构与DNA捕获探头和AuNPs助理探头或SERS纳米探头.
- 通过检测人类血清样本中的目标miRNA来验证生物传感器的性能.
主要成果:
- SPRi模式实现了三种miRNA的同时检测,其检测极限 (LOD) 低,范围从4.6 fM到6.3 fM,动态范围广 (500 pM10 nM).
- SERS模式表现出极高的灵敏度,LOD低至0.8 fM,恢复率高 (90.0%100.2%).
- 双模式方法提供了冗余信号,提高了数据可靠性,并最大限度地减少了假阳性/假阴性.
结论:
- 开发的SPRi/SERS双模式生物传感器为早期癌症检测提供了一个高度敏感,特定和可靠的平台.
- 使用AuNPs显著增强SPRi和SERS信号,改善检测能力.
- 这项技术对在早期疾病阶段检测癌症相关核酸的临床应用具有很大的前景.
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