在Antheraea pernyi中的Peroxiredoxin-2基因调节免疫功能并保护DNA损伤
Isma Gul1, Muhammad Nadeem Abbas2, Najibullah Hussaini1
1State Key Laboratory of Resource Insects, Southwest University, Chongqing 400716, China; Chongqing Engineering and Technology Research Center for Silk Biomaterials and Regenerative Medicine, Chongqing 400716, China.
International journal of biological macromolecules
|November 29, 2023
概括
昆虫 (Antheraea pernyi peroxiredoxin 2) (ApPrx-2) 保护昆虫免受氧化应激和DNA损伤的影响. 这种过氧化素在昆虫的免疫力和生存中起着关键作用.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 氧化素对昆虫防御氧化损伤和免疫反应至关重要.
- 了解特定的过氧化素功能,例如Antheraea pernyi中的ApPrx-2,对于昆虫生理学研究至关重要.
研究的目的:
- 为了克隆和描述Antheraea pernyi peroxiredoxin 2 (ApPrx-2) 基因.
- 调查ApPrx-2在氧化应激防御和A. pernyi. 内的免疫调节中的功能性作用.
主要方法:
- 基因克隆和ApPrx-2的特征.
- 定量实时PCR分析mRNA表达水平,特别是在血细胞中.
- 免疫挑战测试用于评估转录诱导后微生物挑战.
- 使用重组ApPrx-2 (rApPrx-2) 的DNA裂变测试来评估对氧化应激的保护.
- 评估抗氧化活性 (H2O2去除) 和ApPrx-2敲击对幼虫H2O2水平,生存和抗菌活性的影响.
主要成果:
- ApPrx-2基因具有687bp的开放读取框架,编码288氨基酸蛋白质,在血细胞中mRNA水平最高.
- ApPrx-2转录是由微生物挑战诱导的,rApPrx-2保护DNA免受氧化损伤.
- rApPrx-2通过去除H2O2.2,显示出显著的抗氧化活性.
- 在幼虫中抑制ApPrx-2导致H2O2水平升高,生存率降低,抗菌活性显著增加.
结论:
- ApPrx-2在保护Antheraea pernyi免受氧化应激,包括保护DNA方面发挥着重要作用.
- 该研究表明,ApPrx-2参与调节抗菌活性,其耗尽增强了这一功能.
- ApPrx-2涉及A. pernyi内的各种生理功能,有助于氧化防御和免疫调节.
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