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PHRF1通过调节肠直肠癌 HCT116-p53细胞中的SOX4表达来促进细胞入侵

Hung-Wei Lin¹, Ting-Wei Shih¹, Adaobi Amanna²

¹Institute of Biochemical Sciences, National Taiwan University, Taipei, Taiwan, R.O.C.

Anticancer research

|November 29, 2023

概括

含有PHD和RING指域的蛋白1 (PHRF1) 通过调节Kras突变和p53损失的细胞中的SOX4表达来影响结直肠癌的侵袭. 这项研究揭示了PHRF1

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症遗传学癌症遗传学

背景情况:

众所周知,PHRF1 (PHD和RING指域含有蛋白1) 可以在任何地方化TGIP并增强TGF-β信号传递.
它在癌症侵袭中的作用,特别是在Kras突变和p53失活的背景下,仍然在很大程度上未被描述.

研究的目的:

为了研究PHRF1在结直肠癌细胞入侵中的功能.
确定PHRF1在存在瘤性Kras (KrasG13D) 和p53缺陷 (p53-/-) 的情况下对细胞入侵的影响.

主要方法:

使用CRISPR-Cas9基因编辑,在HCT116-p53-/- (KrasG13D/p53-/-) 大肠直肠癌细胞中淘汰PHRF1.
在PHRF1-淘汰细胞上进行表达概况和入侵试验.

主要成果:

与肺癌细胞不同,PHRF1淘汰并没有影响HCT116-p53-/-细胞中的Zeb1表达.
发现SOX4表达对于PHRF1-KNOCK-OUT HCT116-p53-/-细胞中观察到的受损入侵是关键的.
PHRF1的C端SRI域对于入侵和SOX4表达至关重要;重新引入SOX4部分恢复了入侵.

结论:

在克拉斯突变,p53缺乏结直肠癌细胞的入侵中,PHRF1起着重要作用.
通过调节SOX4表达,PHRF1调节结直肠癌细胞的入侵.

关键词:

HCT116 一个国家这是一个很棒的体验,很棒的体验.在 PHRF1 的情况下,PHRF1 是 PHRF1.这就是SOX4的SOX4.在 p53 里,p53 是一个 p53 的代码.

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