8-腺诱导ER压力和细胞死亡在胆管癌细胞中
Jomnarong Lertsuwan1, Jisnuson Svasti2, Jutamaad Satayavivad3
1Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand.
Anticancer research
|November 29, 2023
概括
8-腺 (8-CA) 通过诱导内细胞网膜应激和亡,有效地抑制胆管癌的生长. 在体内研究表明,当单独用于治疗胆管癌瘤时,8-CA最有效.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 胆管癌 (CCA) 是一种致命的癌症,有效的化疗选择有限.
- 在CCA细胞中的腺素敏感性表明潜在的治疗点.
- 8-腺 (8-CA) 是一种腺类型,具有强大的抗白血病作用.
研究的目的:
- 为了研究8-CA对胆管癌细胞和永生胆管细胞的影响.
- 确定8-CA诱导细胞死亡的机制及其对细胞过程的影响.
- 在临床前模型中评估单独和与其他药物结合使用8-CA的疗效.
主要方法:
- 使用MTT测试评估的细胞活力.
- 通过跨井检测分析的入侵.
- 细胞循环,细胞亡,RNA/蛋白质合成和线粒体潜力通过流细胞计和色度测试进行评估.
- 西方涂抹用于蛋白质水平分析;体内有效性在小鼠中进行了测试.
主要成果:
- 8-CA显著降低了胆管癌细胞生长,殖民地形成,并通过内细胞网膜应激诱导细胞循环停止和细胞亡.
- 8-CA没有影响RNA/蛋白质合成或线粒体膜潜力.
- 与8-CA联合治疗的效果通常低于单独使用的8-CA,除了在体外对氧化的添加效果外;然而,在体内,8-CA单一治疗的效果优于联合治疗.
结论:
- 8-腺有效地抑制胆管癌细胞的增殖,并通过内细胞网膜应激诱导细胞亡.
- 临床前数据表明,8-CA单一疗法是抑制胆管癌瘤瘤生长的优越策略,与含氧化的组合疗法相比.
关键词:
5-FU的使用情况.8 - 氨基氨酸是什么?在CX-494545中使用.胆性瘤是一种胆性瘤.压力ERER压力ERER压力灭症 (apoptosis) 是一种死亡的过程.西斯普拉丁 (cisplatin) 是一种制剂.杰姆西塔宾 (gemcitabine) 是一种药物.氧化chloroquine 的使用情况帕克利塔克塞尔 (paclitaxel) 是一种药物.更多相关视频
10:50Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
Published on: April 24, 2021
1.0K
19:44Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
Published on: May 30, 2012
18.7K
相关概念视频
01:31The Intrinsic Apoptotic Pathway
6.6K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
01:46Electron Transport Chain: Complex I and II
14.0K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
14.0K