相关实验视频

Updated: Aug 13, 2026

Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators

Quantum State Engineering of Light with Continuous-wave Optical Parametric Oscillators

Published on: May 30, 2014

基于时间分辨率和模式选择性单光子检测的量子光学断层扫描,由femtosecond上转换检测

Naoto Namekata¹, Nobuaki Kobayashi², Kenya Nomura³

¹Institute of Quantum Science, Nihon University, 1-8-14 Kanda-Surugadai, Chiyoda-Ku, Tokyo, 101-8308, Japan. namekata.naoto@nihon-u.ac.jp.

Scientific reports

|November 29, 2023

概括

我们使用专用光子探测器创建了一个用于3D脑部成像的新型光学系统. 这种方法可以实现高灵敏度和高分辨率的非侵入性生物样本成像.

科学领域:

生物医学光学生物医学光学
神经成像是一种神经成像.
光子学是一个光子学.

背景情况:

光学成像技术对于非侵入性生物样本分析至关重要.
现有的方法在灵敏度,分辨率或辐射水平方面存在局限性.
先进的光子检测是提高断层成像能力的关键.

研究的目的:

开发一种光学飞行时间测量系统,用于高分辨率的小鼠脑断层成像.
为了提高灵敏度,使用时间分辨率和模式选择性的上转换单光子探测器.
为了实现无接触,无侵袭的3D结构成像与最小的光学辐射.

主要方法:

在1556 nm使用100 femt秒模式锁定光纤激光器来产生探头和脉冲.
采用4f系统进行脉冲的光谱雕刻,分隔30nm.
集成了一个具有时间分辨率和模式选择性的上转换单光子探测器用于数据采集.

主要成果:

获得了111dB的灵敏度,相当于射击噪声有限的光学连贯性断层扫描.
通过380 femtosecond脉冲获得了57μm的轴分辨率.
用超弱光学辐射 (1.5mW探测器,30μW) 演示成像.

结论:

开发的光学飞行时间系统为生物成像提供了高性能.
这种技术为标本的非侵入性3D结构成像提供了新的途径.
该系统的灵敏度和分辨率为先进的神经成像应用铺平了道路.

更多相关视频

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

相关概念视频

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...